FastQCFastQC Report
Thu 26 May 2016
SRR1512592_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512592_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010299
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT92490.46008081384908417No Hit
GTCCTAAAGTGTGTATTTCTCATTT81570.4057605361192539No Hit
CTGTAGGACGTGGAATATGGCAAGA80450.4001892255828611No Hit
CTTTAGGACGTGAAATATGGCGAGG68320.33984994271996355No Hit
GTCCTACAGTGTGCATTTCTCATTT51190.254638737819598No Hit
GTACATGGGAAGCAGTGGTATCAAC38290.1904691789629304No Hit
CTGAAGGACCTGGAATATGGCGAGA37410.186091720684336No Hit
GTATCAACGCAGAGTACTTTTTTTT35840.17828193716457105No Hit
CTGTAGGACCTGGAATATGGCGAGA34330.1707706167092557No Hit
GTCCTTCAGTGTGCATTTCTCATTT31770.15803619262607205No Hit
ATTTAGAAATGTCCACTGTAGGACG31170.15505156198157588No Hit
CCCATGTACTCTGCGTTGATACCAC29070.1446053547258393No Hit
TTTCTAAATTTTCCACCTTTTTCAG27510.13684531505014924No Hit
GAATATGGCAAGAAAACTGAAAATC27230.13545248741605104No Hit
GGAATATGGCGAGAAAACTGAAAAT23300.11590315669460115No Hit
GGTATCAACGCAGAGTACTTTTTTT21540.10714824013741239No Hit
TATCAACGCAGAGTACTTTTTTTTT21080.10486002330996533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2400.014.59254419
TCCAACG3650.013.73986418
TAGGACC14500.013.7219344
GTCCTAT3600.012.8249181
GTGTAGG3650.012.374251
TGGCGAA1155.6516E-912.34220718
GGCGAGG20550.011.7914919
TAGAAAT12800.011.7693164
ACCTTTT10900.011.74026315
GAAATGT12200.011.7274316
AGGACCT27900.011.7159885
CACCTTT11150.011.56204314
CGTTTCC2550.011.52370314
AAATGTC12350.011.508277
ATTTAGA11800.011.4828851
AATGTCC12500.011.3423628
GGACCTG27350.011.32866
CCACCTT11000.011.19817613
TTTAGAA14000.011.1668542
GCGCCAC1201.3223689E-711.0546113
Produced by FastQC (version 0.11.5)