FastQCFastQC Report
Thu 26 May 2016
SRR1512592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512592_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010299
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT99410.49450355394893997No Hit
CTGTAGGACGTGGAATATGGCAAGA94210.46863675502997315No Hit
GTCCTAAAGTGTGTATTTCTCATTT88510.44028276390725957No Hit
CTTTAGGACGTGAAATATGGCGAGG81490.4053625853666544No Hit
GTCCTACAGTGTGCATTTCTCATTT56210.2796101475452159No Hit
GTATCAACGCAGAGTACTTTTTTTT47390.23573607707112224No Hit
CTGAAGGACCTGGAATATGGCGAGA46190.2297668157821299No Hit
GTACATGGGAAGCAGTGGTATCAAC45590.22678218513763376No Hit
CTGTAGGACCTGGAATATGGCGAGA40810.20300462766981428No Hit
ATTTAGAAATGTCCACTGTAGGACG35370.17594397649304905No Hit
TTTCTAAATTTTCCACCTTTTTCAG34180.17002445904813165No Hit
CCCATGTACTCTGCGTTGATACCAC33990.16907932601070785No Hit
GTCCTTCAGTGTGCATTTCTCATTT33200.1651495623287879No Hit
GGTATCAACGCAGAGTACTTTTTTT30480.15161923674040528No Hit
GAATATGGCAAGAAAACTGAAAATC30210.15027615295038202No Hit
TATCAACGCAGAGTACTTTTTTTTT29870.14858486225183418No Hit
GGAATATGGCGAGAAAACTGAAAAT27860.13858634959277202No Hit
ATTCCAGGTCCTTCAGTGTGCATTT24080.11978317653244616No Hit
TTGTAGAACAGTGTATATCAATGAG23560.11719649664054949No Hit
GCCATATTCCACGTCCTACAGTGGA22980.1143113536842032No Hit
TTCCAGGTCCTTCAGTGTGCATTTC22790.11336622064677941No Hit
ACAGTGGACATTTCTAAATTTTCCA22590.1123713437652807No Hit
ACCTGGAATATGGCGAGAAAACTGA21850.10869029930373542No Hit
GATATACACTGTTCTACAAATCCCG20440.10167641728916943No Hit
GACCTGGAATATGGCGAGAAAACTG20170.10033333349914615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3400.015.64156918
CCAACGA2200.015.53922719
TAGGACC15250.013.8336034
CCAACGT1501.8189894E-1212.66159319
GTCCTAG2950.012.25799751
CCACCTT11850.012.183513
CTAGGAC3450.012.1189263
TAGGACT4500.012.0368974
GTCCTAT3250.012.0048971
CCGTTTC2000.011.87290113
ACCTTTT12150.011.80302915
CACCTTT12350.011.68936814
TTCCACC11650.011.49639311
GGCGAGG22650.011.4876719
CCGGTTT2400.011.47742412
GCGTGCG752.0763604E-411.3988369
GCCGGTT2500.011.39855211
TGGCGAA1501.7826096E-1011.39600118
AGGACCT31050.011.1707785
CGTTTCC2150.011.04373514
Produced by FastQC (version 0.11.5)