FastQCFastQC Report
Thu 26 May 2016
SRR1512591_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512591_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186182
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT65180.29814535111898277No Hit
GTCCTAAAGTGTGTATTTCTCATTT61400.28085493339529827No Hit
CTGTAGGACGTGGAATATGGCAAGA57640.26365599936327355No Hit
CTTTAGGACGTGAAATATGGCGAGG51560.23584495709872277No Hit
GTATCAACGCAGAGTACTTTTTTTT48000.21956085998329505No Hit
GTCCTACAGTGTGCATTTCTCATTT35540.16256652007929806No Hit
GGTATCAACGCAGAGTACTTTTTTT29230.13370341536066072No Hit
TATCAACGCAGAGTACTTTTTTTTT27810.1272080732528216No Hit
CTGTAGGACCTGGAATATGGCGAGA27390.12528691572796777No Hit
CTGAAGGACCTGGAATATGGCGAGA26930.1231827908197945No Hit
GTCCTTCAGTGTGCATTTCTCATTT23150.10589237309611002No Hit
ATTTAGAAATGTCCACTGTAGGACG22120.10118096297563514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11950.012.9227254
TGTCGAG2300.011.93123618
GTCCTAG3600.011.7147581
GTCGAGG1800.011.56818519
TCCAACG1800.011.56552818
GGCGAGG16750.011.52737619
GTGTAGG4850.011.3869511
AGGACCT22600.011.3604435
CCACCTT9400.011.08942713
GGACCTG21700.011.0460026
ACCGTCC600.006112810711.0248798
CACCTTT9950.010.86115314
ACCTTTT9950.010.86115315
TTTAGAA9950.010.8551582
TTAGGAC23950.010.7601363
AGGACGT39750.010.677645
GTCCAGA3300.010.6497791
AAATGTC8700.010.5632337
TAGAAAT9900.010.52669054
GGACGTG40100.010.4901816
Produced by FastQC (version 0.11.5)