##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512591_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2186182 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.13734629596255 31.0 25.0 33.0 2.0 34.0 2 27.7586275982512 31.0 26.0 34.0 16.0 34.0 3 28.873692583691568 31.0 28.0 34.0 16.0 34.0 4 32.6443877957096 35.0 33.0 37.0 25.0 37.0 5 32.954181307869156 35.0 35.0 37.0 26.0 37.0 6 33.16019709246531 37.0 35.0 37.0 27.0 37.0 7 33.135472252538904 37.0 35.0 37.0 27.0 37.0 8 33.1516996297655 37.0 35.0 37.0 27.0 37.0 9 34.61052968142634 39.0 35.0 39.0 27.0 39.0 10 34.51293899592989 39.0 35.0 39.0 27.0 39.0 11 34.553166204826496 39.0 35.0 39.0 27.0 39.0 12 34.47978850800162 39.0 35.0 39.0 27.0 39.0 13 34.453317701819884 39.0 35.0 39.0 27.0 39.0 14 35.518809047005234 40.0 36.0 41.0 26.0 41.0 15 35.448736655960026 40.0 36.0 41.0 25.0 41.0 16 35.30095801721906 40.0 36.0 41.0 25.0 41.0 17 35.247957397874465 40.0 36.0 41.0 25.0 41.0 18 35.20332936599057 40.0 36.0 41.0 24.0 41.0 19 35.213251687187984 40.0 36.0 41.0 24.0 41.0 20 35.01533495381446 40.0 36.0 41.0 19.0 41.0 21 34.983871425160395 40.0 36.0 41.0 20.0 41.0 22 34.89399464454469 40.0 36.0 41.0 19.0 41.0 23 34.73830083680133 40.0 35.0 41.0 18.0 41.0 24 34.62029785260331 40.0 35.0 41.0 18.0 41.0 25 34.483663757180324 40.0 35.0 41.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.26690059309899894 1101 2 -2.0269938312259796 1101 3 -2.5233305170584153 1101 4 -3.006270204306201 1101 5 -3.2871039242138274 1101 6 -3.277914074730507 1101 7 -3.1816904790078766 1101 8 -3.554726759123472 1101 9 -3.6558213259146903 1101 10 -3.896882433387187 1101 11 -4.062080804478363 1101 12 -3.9244890708046434 1101 13 -4.146282296340299 1101 14 -4.965339188642208 1101 15 -4.64564435124802 1101 16 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1103 3 0.2057883374616054 1103 4 -0.05524812919045985 1103 5 -0.22023854188428515 1103 6 -0.1888181395630184 1103 7 -0.522844431834244 1103 8 -0.4680781963070757 1103 9 -0.38589413752797697 1103 10 -0.6851832011245449 1103 11 -0.7034325586317323 1103 12 -0.7711214193982414 1103 13 -0.6303103725471431 1103 14 -0.7944345752081716 1103 15 -0.8232144731899496 1103 16 -0.8327401881596757 1103 17 -1.0216167545906956 1103 18 -1.0172982401315807 1103 19 -0.9933638233837883 1103 20 -0.9103806597968429 1103 21 -0.864391529247122 1103 22 -1.1033654511898732 1103 23 -1.1772760753989147 1103 24 -1.2821203781410233 1103 25 -1.2036419479017084 1104 1 1.9825372488479545 1104 2 1.1127995947699247 1104 3 0.05951243547561447 1104 4 -0.46157546387768633 1104 5 -0.5479196260939929 1104 6 -0.4949484483446298 1104 7 -0.753806227752996 1104 8 -0.6032279079090941 1104 9 -1.0016809552411061 1104 10 -0.8181754120569948 1104 11 -0.8510409967668267 1104 12 -0.936705419565655 1104 13 -0.8819858637397218 1104 14 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0.4208539915237566 1107 12 0.2605620142346794 1107 13 0.23989270829493847 1107 14 0.25410057802508845 1107 15 0.22449227852009557 1107 16 0.1357774465382775 1107 17 0.20451108794428308 1107 18 0.19358734339704853 1107 19 0.08472874006743325 1107 20 0.300145559787687 1107 21 0.1202207113907221 1107 22 0.11503332470612548 1107 23 0.09120553150994226 1107 24 0.046082361616058165 1107 25 -0.10964944921276754 1108 1 -3.4674834284126064 1108 2 -4.884934372082238 1108 3 -4.458220480485188 1108 4 -3.360291333018356 1108 5 -2.1896043995539287 1108 6 -1.5338250065586578 1108 7 -1.1584459131083413 1108 8 -1.087899781677585 1108 9 -1.2888060765071572 1108 10 -1.2182717437650084 1108 11 -1.2018777690123983 1108 12 -1.0723435782093844 1108 13 -1.1299281114211936 1108 14 -1.5420809528312631 1108 15 -1.4552746795620095 1108 16 -1.5299690152494136 1108 17 -1.411341614681163 1108 18 -1.6076654555379264 1108 19 -1.3483870086712884 1108 20 -1.4562618818186053 1108 21 -1.4974341613964128 1108 22 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0.4406084550149245 2316 17 0.4307626349999083 2316 18 0.44201488657807175 2316 19 0.4279472940295932 2316 20 0.546790933030735 2316 21 0.5040497222298868 2316 22 0.6054961617980865 2316 23 0.5973984528698679 2316 24 0.39048734907684945 2316 25 0.41757144302172833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 108195.0 3 16.0 4 981.0 5 3947.0 6 4536.0 7 4297.0 8 3787.0 9 3645.0 10 3555.0 11 3529.0 12 3608.0 13 3703.0 14 3765.0 15 3709.0 16 3867.0 17 4189.0 18 4697.0 19 5509.0 20 7344.0 21 8772.0 22 10624.0 23 12509.0 24 14369.0 25 16256.0 26 18759.0 27 20881.0 28 24201.0 29 29329.0 30 35825.0 31 45776.0 32 59902.0 33 79831.0 34 110940.0 35 160433.0 36 247531.0 37 326048.0 38 787317.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.446299978941646 20.36197026443555 12.233834308194131 24.957895448428673 2 13.953014009256387 20.211869204158912 43.2550172371196 22.580099549465103 3 18.573013006773788 25.187677021366305 30.02598604911226 26.213323922747644 4 11.973881932513356 17.15657138038698 39.37617633728382 31.49337034981584 5 12.540799439941209 38.35193170801438 36.68476717058519 12.422501681459233 6 31.01678995435674 37.94487511477284 18.549399651521465 12.488935279348956 7 27.290506090455935 31.712258394614512 23.111949474086455 17.885286040843095 8 23.91790045069513 38.88332758342264 20.97163703627582 16.227134929606414 9 26.500934085737793 15.02965504401713 20.85377362383996 37.61563724640512 10 14.909201934238107 29.070509470395045 35.6693916172013 20.350896978165554 11 33.65661299912487 21.954824279714842 26.244881137878412 18.143681583281875 12 23.656345818112463 24.486570793539016 32.98261153411675 18.874471854231768 13 29.321809608178672 22.50647900033564 25.932554202592335 22.23915718889335 14 20.12963071662839 23.18550014307621 28.09704969513761 28.587819445157788 15 22.165124461084744 33.038798227006666 23.288619927174732 21.507457384733865 16 20.98255104888161 28.248848328772315 29.883046930631256 20.88555369171482 17 21.07022488827305 29.45550437690129 28.86175740827494 20.61251332655072 18 20.83066875890197 27.417725963934153 31.954585107997207 19.797020169166668 19 23.52267185844342 26.757449429674523 28.01524534445019 21.704633367431867 20 23.446620651475023 27.850632873105035 28.90529153317113 19.79745494224881 21 22.18535427151802 27.076125519100135 27.64184153351118 23.096678675870667 22 22.757895615628897 29.36906174614307 27.664734438237115 20.208308199990917 23 21.245524152504895 28.850328759677524 28.860777941593657 21.043369146223927 24 23.06340128513142 27.111464319341028 29.620012590690596 20.205121804836953 25 22.19618917861871 27.945243083544646 28.984891857798377 20.873675880038267 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1789.0 1 1789.0 2 1698.0 3 1607.0 4 1607.0 5 1607.0 6 2564.5 7 3522.0 8 3522.0 9 3522.0 10 4643.0 11 5764.0 12 5764.0 13 5764.0 14 9199.5 15 12635.0 16 12635.0 17 12635.0 18 21721.5 19 30808.0 20 30808.0 21 30808.0 22 48778.0 23 66748.0 24 66748.0 25 66748.0 26 96243.5 27 125739.0 28 125739.0 29 125739.0 30 161601.5 31 197464.0 32 197464.0 33 197464.0 34 217162.5 35 236861.0 36 236861.0 37 236861.0 38 247943.5 39 259026.0 40 259026.0 41 259026.0 42 266769.0 43 274512.0 44 274512.0 45 274512.0 46 276192.0 47 277872.0 48 277872.0 49 277872.0 50 259173.5 51 240475.0 52 240475.0 53 240475.0 54 208235.0 55 175995.0 56 175995.0 57 175995.0 58 149276.5 59 122558.0 60 122558.0 61 122558.0 62 99307.0 63 76056.0 64 76056.0 65 76056.0 66 58596.5 67 41137.0 68 41137.0 69 41137.0 70 30297.5 71 19458.0 72 19458.0 73 19458.0 74 13808.5 75 8159.0 76 8159.0 77 8159.0 78 5821.5 79 3484.0 80 3484.0 81 3484.0 82 2597.0 83 1710.0 84 1710.0 85 1710.0 86 1397.0 87 1084.0 88 1084.0 89 1084.0 90 912.0 91 740.0 92 740.0 93 740.0 94 635.0 95 530.0 96 530.0 97 530.0 98 489.5 99 449.0 100 449.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.94602828126844 2 0.20826262406332136 3 0.19403691001023704 4 0.18712989128992918 5 0.16256652007929806 6 0.2149409335544799 7 0.40815449033977963 8 0.2944860034525945 9 0.24883564131440108 10 0.22221388704142656 11 0.21905769967916672 12 0.22715400639105068 13 0.24197436443992312 14 0.25276944005576846 15 0.33245173549137264 16 0.4969851549413544 17 0.5037549481241727 18 0.21878324860418757 19 0.1953176816934729 20 0.1897829183480607 21 0.2068446268425959 22 0.28099215893278784 23 0.19179555956457423 24 0.31077010056802223 25 0.35468227256468127 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2186182.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.88274702298826 #Duplication Level Percentage of deduplicated Percentage of total 1 86.53755736068061 63.07094901105821 2 9.27077028680703 13.51358411043186 3 2.2657631589841776 4.954051293907516 4 0.7690765480792625 2.242096459798958 5 0.3610359633655101 1.3156646392084665 6 0.19093174412424727 0.8349378003399265 7 0.12008982001730244 0.6126733180650071 8 0.08430592614046241 0.4915557989947244 9 0.056142511538669615 0.36826384191372513 >10 0.3005340276030988 3.901818933029358 >50 0.019993629721520655 1.008695065323301 >100 0.019371649043060754 2.9108877351595535 >500 0.002087190550518729 1.056728189861794 >1k 0.0020871905505180947 2.6312181590166492 >5k 2.5299279400219326E-4 1.0868756438909346 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6518 0.29814535111898277 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6140 0.28085493339529827 No Hit CTGTAGGACGTGGAATATGGCAAGA 5764 0.26365599936327355 No Hit CTTTAGGACGTGAAATATGGCGAGG 5156 0.23584495709872277 No Hit GTATCAACGCAGAGTACTTTTTTTT 4800 0.21956085998329505 No Hit GTCCTACAGTGTGCATTTCTCATTT 3554 0.16256652007929806 No Hit GGTATCAACGCAGAGTACTTTTTTT 2923 0.13370341536066072 No Hit TATCAACGCAGAGTACTTTTTTTTT 2781 0.1272080732528216 No Hit CTGTAGGACCTGGAATATGGCGAGA 2739 0.12528691572796777 No Hit CTGAAGGACCTGGAATATGGCGAGA 2693 0.1231827908197945 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2315 0.10589237309611002 No Hit ATTTAGAAATGTCCACTGTAGGACG 2212 0.10118096297563514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.574184582985314E-5 0.0 5 0.0 0.0 0.0 4.574184582985314E-5 0.0 6 0.0 0.0 0.0 4.574184582985314E-5 0.0 7 0.0 0.0 0.0 9.148369165970628E-5 0.0 8 0.0 0.0 0.0 9.148369165970628E-5 0.0 9 0.0 0.0 0.0 1.372255374895594E-4 0.0 10 0.0 0.0 0.0 2.287092291492657E-4 0.0 11 0.0 0.0 0.0 2.744510749791188E-4 0.0 12 0.0 0.0 0.0 2.744510749791188E-4 0.0 13 0.0 0.0 0.0 2.744510749791188E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1195 0.0 12.922725 4 TGTCGAG 230 0.0 11.931236 18 GTCCTAG 360 0.0 11.714758 1 GTCGAGG 180 0.0 11.568185 19 TCCAACG 180 0.0 11.565528 18 GGCGAGG 1675 0.0 11.527376 19 GTGTAGG 485 0.0 11.386951 1 AGGACCT 2260 0.0 11.360443 5 CCACCTT 940 0.0 11.089427 13 GGACCTG 2170 0.0 11.046002 6 ACCGTCC 60 0.0061128107 11.024879 8 CACCTTT 995 0.0 10.861153 14 ACCTTTT 995 0.0 10.861153 15 TTTAGAA 995 0.0 10.855158 2 TTAGGAC 2395 0.0 10.760136 3 AGGACGT 3975 0.0 10.67764 5 GTCCAGA 330 0.0 10.649779 1 AAATGTC 870 0.0 10.563233 7 TAGAAAT 990 0.0 10.5266905 4 GGACGTG 4010 0.0 10.490181 6 >>END_MODULE