FastQCFastQC Report
Thu 26 May 2016
SRR1512591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186182
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA70880.32421820324199907No Hit
GTCCTACAGTGGACATTTCTAAATT70830.3239894940128498No Hit
GTCCTAAAGTGTGTATTTCTCATTT65130.2979166418898335No Hit
CTTTAGGACGTGAAATATGGCGAGG61390.28080919154946843No Hit
GTATCAACGCAGAGTACTTTTTTTT60330.27596055589150403No Hit
GGTATCAACGCAGAGTACTTTTTTT40350.18456834792345742No Hit
TATCAACGCAGAGTACTTTTTTTTT39960.18278441593609315No Hit
GTCCTACAGTGTGCATTTCTCATTT38640.17674649228655254No Hit
CTGAAGGACCTGGAATATGGCGAGA33750.15437872967575433No Hit
CTGTAGGACCTGGAATATGGCGAGA31610.14458997466816578No Hit
TTTCTAAATTTTCCACCTTTTTCAG25340.11590983733284785No Hit
ATTTAGAAATGTCCACTGTAGGACG24850.11366848688718506No Hit
GTCCTTCAGTGTGCATTTCTCATTT24480.11197603859148049No Hit
GAATATGGCAAGAAAACTGAAAATC22960.10502327802534282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG402.7564625E-416.6291854
TAGGACC10600.013.08824
TCCAACG2200.012.517858518
CCAACGA1251.4206307E-912.15527219
ACGATGT658.024168E-411.6925758
ATCCCGT1157.076051E-811.56548110
GGCGAGG16200.011.547976519
GTACTAG1657.2759576E-1211.5272851
GCCGGTT1604.5474735E-1111.28150811
CAGGACG1104.9549635E-711.23014
GTAAGAC1900.011.0025183
GCACCGT951.3583705E-511.0017626
AGGACCT20200.010.8678415
GTCCTAG3600.010.8308451
GTCCTAA19150.010.7266941
GGACGTG36300.010.6793976
AGGACGT37100.010.6561935
TGTAGGA36150.010.6193042
TCTAGAC1803.45608E-1110.5579723
GTCCTAC34050.010.5015091
Produced by FastQC (version 0.11.5)