FastQCFastQC Report
Thu 26 May 2016
SRR1512590_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512590_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168390
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT61520.283712800741564No Hit
GTCCTAAAGTGTGTATTTCTCATTT60110.277210280438482No Hit
CTGTAGGACGTGGAATATGGCAAGA57120.26342124802272654No Hit
CTTTAGGACGTGAAATATGGCGAGG49450.2280493822605712No Hit
GTATCAACGCAGAGTACTTTTTTTT45790.21117049977172003No Hit
GTCCTACAGTGTGCATTTCTCATTT35130.16200960159380923No Hit
TATCAACGCAGAGTACTTTTTTTTT29740.13715244951323333No Hit
GGTATCAACGCAGAGTACTTTTTTT28370.13083439787123166No Hit
CTGAAGGACCTGGAATATGGCGAGA26000.11990462970222146No Hit
CTGTAGGACCTGGAATATGGCGAGA23030.10620783161700617No Hit
ATTTAGAAATGTCCACTGTAGGACG22550.10399420768404208No Hit
GTCCTTCAGTGTGCATTTCTCATTT22310.10288739571756003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC905.573893E-712.63517813
TAGGACC10600.012.5136554
TCCAACG2200.012.04743618
AATCCCG2350.011.2800219
GGCGAGG15700.011.2761719
TGTCCAC10000.011.17487210
GTATTAG2000.011.0256291
GTCCTAT3550.011.0115111
AATGTCC9300.010.9760598
AGGACCT21950.010.5753215
CACCTTT8300.010.39531614
GTGTAGG4050.010.3945331
CCAACGT1201.5934838E-610.25608819
GTCTAGG2350.010.2365611
TGGCGAG35250.010.23116618
ATAAGAC1951.6370905E-1110.2039163
GGACCTG20750.010.1823686
CCACCTT8350.010.10057613
CATTTCT43200.010.09598114
ACCTTTT8550.010.0913615
Produced by FastQC (version 0.11.5)