##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512590_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2168390 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.1631777493901 31.0 25.0 33.0 2.0 34.0 2 27.747864083490516 31.0 27.0 34.0 16.0 34.0 3 28.837726608220844 31.0 28.0 34.0 16.0 34.0 4 32.590111096251135 35.0 33.0 37.0 25.0 37.0 5 32.8928619851595 35.0 35.0 37.0 26.0 37.0 6 33.100228279968086 37.0 35.0 37.0 26.0 37.0 7 33.068917491779615 37.0 35.0 37.0 26.0 37.0 8 33.07814000249033 37.0 35.0 37.0 26.0 37.0 9 34.54135326209769 39.0 35.0 39.0 27.0 39.0 10 34.441627659231045 39.0 35.0 39.0 27.0 39.0 11 34.490352750197154 39.0 35.0 39.0 27.0 39.0 12 34.41296953038891 39.0 35.0 39.0 26.0 39.0 13 34.38325624080539 39.0 35.0 39.0 26.0 39.0 14 35.450722425393955 40.0 36.0 41.0 25.0 41.0 15 35.38115283689742 40.0 36.0 41.0 25.0 41.0 16 35.240704854753986 40.0 36.0 41.0 24.0 41.0 17 35.18695852683327 40.0 36.0 41.0 24.0 41.0 18 35.14798813866509 40.0 36.0 41.0 24.0 41.0 19 35.15700127744548 40.0 36.0 41.0 23.0 41.0 20 34.9583875594335 40.0 36.0 41.0 19.0 41.0 21 34.93345523637353 40.0 36.0 41.0 19.0 41.0 22 34.84144826345814 40.0 36.0 41.0 18.0 41.0 23 34.68551690424693 40.0 35.0 41.0 18.0 41.0 24 34.564100092695504 40.0 35.0 41.0 16.0 41.0 25 34.43486365460088 39.0 35.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.8713470643008314 1101 2 -3.3110627995144277 1101 3 -3.930244350018267 1101 4 -4.6528162626464855 1101 5 -4.956836598448934 1101 6 -4.810322345058651 1101 7 -4.765186794402208 1101 8 -4.943645751214646 1101 9 -5.394072446714837 1101 10 -5.84241708197025 1101 11 -5.851765976349245 1101 12 -5.864742719500203 1101 13 -5.936056156264243 1101 14 -6.879205984083153 1101 15 -6.711641500115412 1101 16 -6.999857506957298 1101 17 -6.990918621097382 1101 18 -7.307484021398924 1101 19 -7.145206178641086 1101 20 -7.13250635616463 1101 21 -7.650479588758145 1101 22 -7.818795487709522 1101 23 -8.205043392019569 1101 24 -8.271137430098694 1101 25 -8.64585248784448 1102 1 0.30880361315241345 1102 2 -0.4065631083240575 1102 3 -0.9210472573160722 1102 4 -1.5653842396626594 1102 5 -1.374424564095861 1102 6 -1.446483265205373 1102 7 -1.5635687176176347 1102 8 -1.5913504466769375 1102 9 -1.7541652226439695 1102 10 -1.9480265937127967 1102 11 -1.9533425853782092 1102 12 -1.8257916665597023 1102 13 -1.874412612550259 1102 14 -2.2230900313090416 1102 15 -2.129109730154802 1102 16 -2.3656910528156985 1102 17 -2.2628808035866257 1102 18 -2.410156290158689 1102 19 -2.4674537084458947 1102 20 -2.494778951525248 1102 21 -2.5307843440656868 1102 22 -2.5418188411137947 1102 23 -2.6153450738876955 1102 24 -2.900594734658501 1102 25 -2.935585148223108 1103 1 2.8308434093792165 1103 2 1.205931823149463 1103 3 0.39479013698210963 1103 4 0.33062931818489716 1103 5 0.0508111684628787 1103 6 -0.1134832873225804 1103 7 -0.2755199344326087 1103 8 -0.08652539377995083 1103 9 -0.2331743349330253 1103 10 -0.5641293944883756 1103 11 -0.6079365134677985 1103 12 -0.6598132750704053 1103 13 -0.4665237736298735 1103 14 -0.6701027339579468 1103 15 -0.8027344085337589 1103 16 -0.8195335488161675 1103 17 -0.7677611494997336 1103 18 -0.8106708838287418 1103 19 -0.8474726555194181 1103 20 -0.9119505663024938 1103 21 -0.9588014996454959 1103 22 -0.7852343644437596 1103 23 -0.9839476719222162 1103 24 -1.0454259435112832 1103 25 -1.0408881170894517 1104 1 1.9026184607940095 1104 2 0.9845982297872915 1104 3 -0.13945566118823294 1104 4 -0.667319408855743 1104 5 -0.794689150663423 1104 6 -0.657100761260601 1104 7 -0.7242700230788017 1104 8 -0.8736521932977155 1104 9 -1.0486572794050488 1104 10 -1.1014955387334595 1104 11 -1.0370355135387186 1104 12 -1.1467840572844281 1104 13 -1.2467343969899218 1104 14 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1107 12 0.5365674871016779 1107 13 0.43126132119181904 1107 14 0.5734558683667146 1107 15 0.2939090475100059 1107 16 0.4126680417412416 1107 17 0.44194682847716393 1107 18 0.5674427582598511 1107 19 0.46474980660588017 1107 20 0.6736147653035403 1107 21 0.5314707303735773 1107 22 0.46655681192098797 1107 23 0.568167373599394 1107 24 0.5674370598353633 1107 25 0.4536888738062217 1108 1 -3.0268827380212784 1108 2 -4.71746467275608 1108 3 -4.147540847592257 1108 4 -3.1356904304344155 1108 5 -1.8712760530187467 1108 6 -1.2464693852086945 1108 7 -0.7904380452686368 1108 8 -0.5098742797064375 1108 9 -1.0091208213165714 1108 10 -0.7351977624182879 1108 11 -0.6189658412148944 1108 12 -0.6630174266605735 1108 13 -0.7318427604895206 1108 14 -1.0724965637046608 1108 15 -0.8984314186270694 1108 16 -0.9977186564147189 1108 17 -0.9856120350453423 1108 18 -1.0347805772204737 1108 19 -0.8952635524914214 1108 20 -0.9280532134173285 1108 21 -0.912527456606071 1108 22 -1.1213503423527271 1108 23 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0.8416856443646523 2316 22 0.8561555813650727 2316 23 0.6770087445426753 2316 24 0.5848749904216106 2316 25 0.6521246812502213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 111384.0 3 8.0 4 960.0 5 3904.0 6 4591.0 7 4266.0 8 3683.0 9 3617.0 10 3430.0 11 3526.0 12 3540.0 13 3599.0 14 3641.0 15 3699.0 16 3862.0 17 4204.0 18 4547.0 19 5573.0 20 7212.0 21 8614.0 22 10466.0 23 12296.0 24 14138.0 25 16020.0 26 18264.0 27 20682.0 28 24184.0 29 29007.0 30 35973.0 31 45062.0 32 59144.0 33 78550.0 34 109426.0 35 158343.0 36 243259.0 37 323313.0 38 782403.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.174477879190455 20.641601865566802 12.203426667149635 24.98049358809311 2 14.015010404119865 20.5283776875769 42.987734138916956 22.468877769386282 3 18.414174397100126 25.474246727524285 30.191482459456235 25.920096415919346 4 11.982459464080254 17.377652913641974 39.58103256273884 31.058855059538935 5 12.522728784619819 38.448510987427106 36.690494933144116 12.338265294808966 6 30.659713267016276 38.271661429893335 18.70087347345515 12.367751829635237 7 27.08002200758039 31.875516381568463 23.461182925970796 17.583278684880348 8 23.97549818748392 38.673023580288266 21.218188446158372 16.13328978606944 9 26.536348625728195 15.080577183523072 21.088458965121983 37.29461522562675 10 14.895819032919954 29.13826806798006 35.6733085297719 20.292604369328078 11 33.41050962700693 22.241656247327757 26.318487412437108 18.029346713228207 12 23.527334317021126 24.67808262237853 33.055931526454174 18.738651534146168 13 29.17512537223313 22.605467787415296 26.06535768824097 22.154049152110606 14 20.13790246212121 23.290876908735793 28.31231319543087 28.25890743371212 15 22.097455234752605 33.1215626602395 23.448233732624715 21.33274837238318 16 20.958824671914307 28.286454236269908 29.90496860176694 20.849752490048846 17 20.959870941722123 29.54878277739533 28.996854668048112 20.494491612834437 18 20.715130610994038 27.581469800671073 31.93399861052626 19.769400977808633 19 23.404039008349095 26.930511699223686 28.19870357268454 21.466745719742676 20 23.32865576733722 28.03319054204473 29.010211941331587 19.627941749286467 21 22.010977573434936 27.14694012619218 27.8690880923462 22.97299420802668 22 22.843889795419983 29.39195814416434 27.796509670281687 19.96764239013398 23 21.198846825466138 28.991785549091016 28.932731331497013 20.876636293945833 24 22.94211136327358 27.31974432113931 29.60012584989162 20.138018465695495 25 22.05993584254112 28.16725377376395 29.044488985377097 20.728321398317835 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1837.0 1 1837.0 2 1755.5 3 1674.0 4 1674.0 5 1674.0 6 2621.0 7 3568.0 8 3568.0 9 3568.0 10 4799.5 11 6031.0 12 6031.0 13 6031.0 14 9678.5 15 13326.0 16 13326.0 17 13326.0 18 22612.5 19 31899.0 20 31899.0 21 31899.0 22 49998.0 23 68097.0 24 68097.0 25 68097.0 26 98211.5 27 128326.0 28 128326.0 29 128326.0 30 164312.5 31 200299.0 32 200299.0 33 200299.0 34 220025.5 35 239752.0 36 239752.0 37 239752.0 38 250451.0 39 261150.0 40 261150.0 41 261150.0 42 267668.5 43 274187.0 44 274187.0 45 274187.0 46 273933.0 47 273679.0 48 273679.0 49 273679.0 50 253507.5 51 233336.0 52 233336.0 53 233336.0 54 201010.0 55 168684.0 56 168684.0 57 168684.0 58 142108.5 59 115533.0 60 115533.0 61 115533.0 62 93508.0 63 71483.0 64 71483.0 65 71483.0 66 55281.5 67 39080.0 68 39080.0 69 39080.0 70 28689.0 71 18298.0 72 18298.0 73 18298.0 74 13159.0 75 8020.0 76 8020.0 77 8020.0 78 5914.0 79 3808.0 80 3808.0 81 3808.0 82 2966.5 83 2125.0 84 2125.0 85 2125.0 86 1762.5 87 1400.0 88 1400.0 89 1400.0 90 1274.0 91 1148.0 92 1148.0 93 1148.0 94 1033.5 95 919.0 96 919.0 97 919.0 98 825.0 99 731.0 100 731.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.786412960768128 2 0.2224692052628909 3 0.20439127647701752 4 0.19645912405056287 5 0.17220149511849805 6 0.2233915485682926 7 0.42123418757695796 8 0.3114753342341553 9 0.26199161589935394 10 0.23017077186299512 11 0.2280493822605712 12 0.23528977720797456 13 0.2504623245818326 14 0.26296007637002566 15 0.3487380037723841 16 0.5120388859937557 17 0.5176651801567062 18 0.22970960021029427 19 0.20489856529498843 20 0.19180129035828428 21 0.21702737976102088 22 0.2906303755320768 23 0.1972431158601543 24 0.32674011593855345 25 0.37262669538228826 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2168390.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.91954778472262 #Duplication Level Percentage of deduplicated Percentage of total 1 85.3391254085098 61.375513077237564 2 10.04020389563064 14.44173847680332 3 2.5200742881201355 5.437278095565207 4 0.8607827535889145 2.4762842551601225 5 0.40465098173184755 1.4551157808399264 6 0.21204733302072426 0.9150208979884179 7 0.12818840275263418 0.6453476370050739 8 0.08564053840484674 0.4927383035293399 9 0.06025404825667957 0.39000995125373233 >10 0.30456456315185976 3.943831434170701 >50 0.021414868006477853 1.0852625233819184 >100 0.018664997431604807 2.7406957986061555 >500 0.0021294324411384354 1.0857716226422058 >1k 0.0020649041853461605 2.6858414638737576 >5k 1.9358476737620258E-4 0.8295506819425548 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6152 0.283712800741564 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6011 0.277210280438482 No Hit CTGTAGGACGTGGAATATGGCAAGA 5712 0.26342124802272654 No Hit CTTTAGGACGTGAAATATGGCGAGG 4945 0.2280493822605712 No Hit GTATCAACGCAGAGTACTTTTTTTT 4579 0.21117049977172003 No Hit GTCCTACAGTGTGCATTTCTCATTT 3513 0.16200960159380923 No Hit TATCAACGCAGAGTACTTTTTTTTT 2974 0.13715244951323333 No Hit GGTATCAACGCAGAGTACTTTTTTT 2837 0.13083439787123166 No Hit CTGAAGGACCTGGAATATGGCGAGA 2600 0.11990462970222146 No Hit CTGTAGGACCTGGAATATGGCGAGA 2303 0.10620783161700617 No Hit ATTTAGAAATGTCCACTGTAGGACG 2255 0.10399420768404208 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2231 0.10288739571756003 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.611716527008518E-5 0.0 9 0.0 0.0 0.0 1.3835149581025553E-4 0.0 10 0.0 0.0 0.0 1.8446866108034072E-4 0.0 11 0.0 0.0 0.0 1.8446866108034072E-4 0.0 12 0.0 0.0 0.0 1.8446866108034072E-4 4.611716527008518E-5 13 0.0 0.0 0.0 2.305858263504259E-4 4.611716527008518E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCCAC 90 5.573893E-7 12.635178 13 TAGGACC 1060 0.0 12.513655 4 TCCAACG 220 0.0 12.047436 18 AATCCCG 235 0.0 11.28002 19 GGCGAGG 1570 0.0 11.27617 19 TGTCCAC 1000 0.0 11.174872 10 GTATTAG 200 0.0 11.025629 1 GTCCTAT 355 0.0 11.011511 1 AATGTCC 930 0.0 10.976059 8 AGGACCT 2195 0.0 10.575321 5 CACCTTT 830 0.0 10.395316 14 GTGTAGG 405 0.0 10.394533 1 CCAACGT 120 1.5934838E-6 10.256088 19 GTCTAGG 235 0.0 10.236561 1 TGGCGAG 3525 0.0 10.231166 18 ATAAGAC 195 1.6370905E-11 10.203916 3 GGACCTG 2075 0.0 10.182368 6 CCACCTT 835 0.0 10.100576 13 CATTTCT 4320 0.0 10.095981 14 ACCTTTT 855 0.0 10.09136 15 >>END_MODULE