FastQCFastQC Report
Thu 26 May 2016
SRR1512590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168390
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66300.3057568057406647No Hit
CTGTAGGACGTGGAATATGGCAAGA65170.30054556606514515No Hit
GTCCTAAAGTGTGTATTTCTCATTT64100.295611029381246No Hit
CTTTAGGACGTGAAATATGGCGAGG61530.2837589179068341No Hit
GTATCAACGCAGAGTACTTTTTTTT56130.2588556486609881No Hit
TATCAACGCAGAGTACTTTTTTTTT38340.1768132116455066No Hit
GGTATCAACGCAGAGTACTTTTTTT38030.17538357952213393No Hit
GTCCTACAGTGTGCATTTCTCATTT37450.172708783936469No Hit
CTGAAGGACCTGGAATATGGCGAGA32340.14914291248345546No Hit
CTGTAGGACCTGGAATATGGCGAGA28200.1300504060616402No Hit
ATTTAGAAATGTCCACTGTAGGACG26620.12276389394896674No Hit
TTTCTAAATTTTCCACCTTTTTCAG25350.11690701395966592No Hit
GTCCTTCAGTGTGCATTTCTCATTT22600.10422479351039252No Hit
GAATATGGCAAGAAAACTGAAAATC21830.10067377178459595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10350.014.4139374
TGGACCG604.0836734E-412.670165
GGCGAGG14150.011.94390119
ACCGTCC658.025744E-411.6922938
CCACCTT8150.011.42171813
GGATAGT1001.927896E-611.4013016
GGACCTG21350.011.3924016
CACCTTT8400.011.30611814
AGGACCT22600.011.18455
GTCCTAT3600.011.102021
CCGTCCA600.005876978411.0833219
ACCTTTT8500.011.06111915
TCCAACG2450.010.851871518
CCAACGA1502.1536835E-910.760694519
TAGAAAT10350.010.7415964
TGTAGGA36100.010.47608952
GTCTAGG2001.8189894E-1210.4676191
TGGCGAG35350.010.39523318
GTAGGAC35300.010.3366513
CTGTAGG36200.010.3309251
Produced by FastQC (version 0.11.5)