FastQCFastQC Report
Thu 26 May 2016
SRR1512589_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512589_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336453
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT67280.2879578574873965No Hit
CTGTAGGACGTGGAATATGGCAAGA59330.2539319215922597No Hit
GTCCTAAAGTGTGTATTTCTCATTT55660.23822435118532234No Hit
CTTTAGGACGTGAAATATGGCGAGG47500.2032996169835216No Hit
GTATCAACGCAGAGTACTTTTTTTT41200.17633566778360188No Hit
GTCCTACAGTGTGCATTTCTCATTT37280.15955809939254073No Hit
CTGAAGGACCTGGAATATGGCGAGA26590.11380498559140716No Hit
CTGTAGGACCTGGAATATGGCGAGA25960.11110859067141518No Hit
TATCAACGCAGAGTACTTTTTTTTT24340.10417500373429296No Hit
GGTATCAACGCAGAGTACTTTTTTT24250.1037898044600084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12550.014.2685384
TACACCG604.1868194E-412.6328165
TCCAACG2000.011.82646418
GTACTAG1551.1823431E-1011.6598151
GATATAC5600.011.4747381
GTCCTAT3700.011.3972061
ACACCGT1001.993376E-611.369786
TGTAGGA37050.011.2782022
GGACCTG22250.011.2420296
CCAACGT1355.0313247E-911.21516819
CGGTTTC1950.011.17831113
AGGATCG855.472378E-511.1466035
AGGACCT23050.011.1393495
TTAACGT600.005995357411.0537154
GTAGGAC36450.010.9957023
CTGTAGG33250.010.9613951
GGCGAGG15850.010.92550319
GTCCTAC32850.010.911481
ATAGGAC4600.010.7108923
CCAACGA909.904993E-510.5142219
Produced by FastQC (version 0.11.5)