FastQCFastQC Report
Thu 26 May 2016
SRR1512589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512589_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2336453
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71680.30678982200797533No Hit
CTGTAGGACGTGGAATATGGCAAGA70120.30011303458704286No Hit
GTCCTAAAGTGTGTATTTCTCATTT61230.2620639062716006No Hit
CTTTAGGACGTGAAATATGGCGAGG57330.24537193771926932No Hit
GTATCAACGCAGAGTACTTTTTTTT54370.23270316158724358No Hit
GTCCTACAGTGTGCATTTCTCATTT38480.16469408971633495No Hit
TATCAACGCAGAGTACTTTTTTTTT36190.154892908181761No Hit
GGTATCAACGCAGAGTACTTTTTTT34690.1484729202770182No Hit
CTGAAGGACCTGGAATATGGCGAGA31700.13567574438689758No Hit
CTGTAGGACCTGGAATATGGCGAGA30850.13203775124087666No Hit
GTCCTTCAGTGTGCATTTCTCATTT23880.10220620744350517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG400.005271634614.2534695
TCCAACG2300.013.21381818
TGCGAGA751.4850881E-512.66188719
TAGGACC12750.012.5204264
TACACCG1052.71335E-711.7647685
GGCGTGC1001.9325635E-611.3991148
GGCGAGG14550.011.35653819
CCAACGA1451.2259989E-911.13372919
TGTAGGA38100.011.0733242
GCCGGTT1303.27218E-810.96068711
AGGACCT23700.010.9457025
GGACCTG23450.010.8572286
GTAGGAC37150.010.6658943
CTGTAGG38150.010.6463311
GTCCTAC37250.010.5971351
CCAACGT1553.6870915E-910.41542319
AGGACGT33350.010.3428485
GGACGTG33500.010.2090366
GTCCTAA18150.010.1669751
TAGAGCG750.002643081610.13585
Produced by FastQC (version 0.11.5)