FastQCFastQC Report
Thu 26 May 2016
SRR1512588_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512588_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747795
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32350.18509035670659316No Hit
GTACATGGGAAGCAGTGGTATCAAC27840.159286415168827No Hit
CCCATGTACTCTGCGTTGATACCAC21780.12461415669457804No Hit
GGTATCAACGCAGAGTACTTTTTTT20780.11889266189684716No Hit
TATCAACGCAGAGTACTTTTTTTTT19950.11414382121473056No Hit
GTCCTACAGTGGACATTTCTAAATT19230.11002434496036435No Hit
CTGTAGGACGTGGAATATGGCAAGA18300.10470335479847465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1505.456968E-1212.7177171
GTCCTAC10800.011.5277551
TATGTCG752.1169303E-411.37492616
TAGGACT2050.011.0923814
TTGGACT2450.010.8282754
GGCCTAC1301.7941056E-710.81263351
GTATAAC2101.8189894E-1210.5184131
GTCCTAA6900.010.4768371
AGGACGT11600.010.4548875
CTTGGAC2001.8189894E-1210.4225163
TTAGGAC8200.010.39940553
CTTATAC1659.731593E-1010.3363783
CGTGAAA7150.010.3144219
TAGGACC3800.010.2227484
GACGTGA6400.010.2149557
ACGTGAA7200.010.1079918
GGCGAGG5350.010.0847219
GTCCTAG2301.8189894E-1210.0403021
GGACGTG11750.09.9988766
TCCTACA12800.09.9934652
Produced by FastQC (version 0.11.5)