FastQCFastQC Report
Thu 26 May 2016
SRR1512588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512588_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747795
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44130.25248956542386264No Hit
GTACATGGGAAGCAGTGGTATCAAC32590.18646351545804857No Hit
GGTATCAACGCAGAGTACTTTTTTT28250.16163222803589666No Hit
TATCAACGCAGAGTACTTTTTTTTT28210.16140336824398743No Hit
CCCATGTACTCTGCGTTGATACCAC24390.13954725811665555No Hit
GTCCTACAGTGGACATTTCTAAATT21820.12484301648648727No Hit
CTGTAGGACGTGGAATATGGCAAGA21400.1224399886714403No Hit
GTCCTAAAGTGTGTATTTCTCATTT19500.11156914855575167No Hit
CTTTAGGACGTGAAATATGGCGAGG17710.10132767286781344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG456.7440234E-414.7832344
TCCAACG1403.6379788E-1212.88765518
CCGTCCA550.003070752112.0898349
GTAAGAC1950.011.6966243
GGCGAGG5300.011.64624319
TTAGACC907.431334E-611.6153974
AGGACCG1001.9212894E-611.4042085
CGGTTTC1201.2784585E-711.08139713
CGAAATC1553.1468517E-1011.03033213
ACGAAAT1553.1468517E-1011.03033212
TAGGACC3200.010.988434
GCCGGTT1252.243687E-710.6387511
AGGACGT12550.010.5257985
CCAACGT1002.4095498E-510.44578419
GTCCTAA6300.010.419941
TTTAGAC1659.222276E-1010.3674623
CTATACT2200.010.3674624
AATACTG2850.010.3371495
AAAGGCG1201.5164333E-610.2954665
TCTACAC2600.010.2345463
Produced by FastQC (version 0.11.5)