FastQCFastQC Report
Thu 26 May 2016
SRR1512587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749557
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT35040.20027927069538173No Hit
GTCCTAAAGTGTGTATTTCTCATTT33200.18976232269082974No Hit
GTATCAACGCAGAGTACTTTTTTTT31970.18273197157909116No Hit
CTGTAGGACGTGGAATATGGCAAGA31070.17758781222903855No Hit
CTTTAGGACGTGAAATATGGCGAGG27330.15621097226326436No Hit
GTCCTACAGTGTGCATTTCTCATTT21780.12448865627127324No Hit
GGTATCAACGCAGAGTACTTTTTTT19640.11225698848337036No Hit
TATCAACGCAGAGTACTTTTTTTTT19610.11208551650503527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1101.2732926E-1114.63012919
TAGGACC6850.013.9712284
GGCGTGC1104.0921805E-812.030038
AGGACCT13450.011.6242635
GGCGAGG9050.011.61091319
TCCAACG1657.2759576E-1211.47296518
GCGCCAC752.1203821E-411.37293113
GGACCTG13500.011.2305876
GTAGGAC21700.010.9165163
GGACGTG21000.010.7843716
TGTAGGA22600.010.6914222
AGGACGT21500.010.6214135
GACGTGG11200.010.3221597
GTCCTAA11000.010.294481
GACGTGA11050.010.2907667
CTGTAGG21950.010.2722771
TAAAGCG750.002705081610.1072385
GTCCTAC18300.010.0759361
CTAGGAC2550.010.032923
TGGCGAG21250.010.02197218
Produced by FastQC (version 0.11.5)