FastQCFastQC Report
Thu 26 May 2016
SRR1512587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1749557
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT39070.22331367311839512No Hit
CTGTAGGACGTGGAATATGGCAAGA37120.21216799452661445No Hit
GTATCAACGCAGAGTACTTTTTTTT36950.21119631998271562No Hit
GTCCTAAAGTGTGTATTTCTCATTT36300.20748109378545537No Hit
CTTTAGGACGTGAAATATGGCGAGG33070.1890192774513777No Hit
GGTATCAACGCAGAGTACTTTTTTT25210.14409361912758487No Hit
TATCAACGCAGAGTACTTTTTTTTT24240.1385493584947504No Hit
GTCCTACAGTGTGCATTTCTCATTT23990.137120425341958No Hit
GTACATGGGAAGCAGTGGTATCAAC19730.11277140441837562No Hit
CTGAAGGACCTGGAATATGGCGAGA18630.10648409854608909No Hit
CTGTAGGACCTGGAATATGGCGAGA18490.10568389598052536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA957.4380296E-812.99339119
GCACCGT905.396505E-712.6685536
GTGCGTT604.0952375E-412.66565611
TAGGACC7450.012.6285714
AAGTCGT1800.011.6115127
AGTCGTC1751.8189894E-1211.3994178
GGCGAGG9800.011.3360719
GATATAC3400.010.9136651
GTCCTAC20350.010.8937241
GGACCTG14200.010.705826
GTCCTAA10700.010.581521
AGGACCT14650.010.5736585
TCCAACG1355.7223588E-810.55109318
TCCTACA21300.010.4848822
TAGAAAT5850.010.3967914
CAAGTCG1659.258656E-1010.3651816
CACCTTT5750.010.23944114
TGGCGAG22550.010.232923518
TAGGACT3100.010.1164364
CCACCTT6200.010.11094113
Produced by FastQC (version 0.11.5)