FastQCFastQC Report
Thu 26 May 2016
SRR1512586_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512586_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2557411
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT57070.22315537080273762No Hit
CTGTAGGACGTGGAATATGGCAAGA49110.19203014298444793No Hit
GTCCTAAAGTGTGTATTTCTCATTT46150.18045593766508394No Hit
CTTTAGGACGTGAAATATGGCGAGG38790.15167683254666536No Hit
GTATCAACGCAGAGTACTTTTTTTT37060.14491217876203707No Hit
GTACATGGGAAGCAGTGGTATCAAC30680.11996507405340792No Hit
GTCCTACAGTGTGCATTTCTCATTT28790.11257478754881402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT552.0068549E-413.7805116
TAGGACC11100.012.3758354
TATGCCG550.003133220612.057715
TCCAACG1603.6379788E-1211.83123118
GGCGAGG11150.011.80102219
GTCCTAG3850.011.7256931
TGTCGAG1407.239578E-1011.493196518
TGTAGGA31250.011.277972
GTAGGAC30600.011.2698473
GTCTTAG3850.011.2045511
TACGACC600.005998904411.0529014
GGACGTG26950.011.0384726
AGGACGT27000.011.0178135
GTCCTAC28200.010.9924911
TAGGACA10300.010.8536124
TATGTCG1502.2100721E-910.74407416
GTCCTAA15450.010.7137671
CTGTAGG28750.010.6775181
ATGTCGA1605.638867E-1010.666121517
CTGCGCA909.9814686E-510.5063339
Produced by FastQC (version 0.11.5)