FastQCFastQC Report
Thu 26 May 2016
SRR1512586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2557411
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT60470.23645006610200706No Hit
CTGTAGGACGTGGAATATGGCAAGA59090.2310539838923036No Hit
GTCCTAAAGTGTGTATTTCTCATTT50060.19574483725924383No Hit
CTTTAGGACGTGAAATATGGCGAGG47790.18686867304473156No Hit
GTATCAACGCAGAGTACTTTTTTTT46960.18362320330990992No Hit
GTACATGGGAAGCAGTGGTATCAAC36300.14194042334220036No Hit
GTCCTACAGTGTGCATTTCTCATTT32790.12821560554795455No Hit
TATCAACGCAGAGTACTTTTTTTTT30260.11832278816349816No Hit
GGTATCAACGCAGAGTACTTTTTTT30030.11742344112854758No Hit
CTGAAGGACCTGGAATATGGCGAGA26960.10541911331420721No Hit
CCCATGTACTCTGCGTTGATACCAC26710.10444156218926094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9950.012.6063764
GGCGAGG12900.011.778254519
ATATCGA658.024382E-411.692686
GAGCCGT658.024382E-411.692686
AGGACGT29300.011.0919115
CGTATTC600.005866615611.0860733
TTAGGAC17500.010.9684253
GGACGTG28750.010.8716526
TGTAGGA31050.010.8335942
GTAGGAC29700.010.7821253
GCGCCAC1252.2450695E-710.63888313
TAGGACG31650.010.5984024
GACGTGA15300.010.555487
CTGTAGG31150.010.41447451
TGGCGAG30300.010.28013518
GTATTAG2053.6379788E-1210.2096251
TAGGACA8750.010.2084364
CGGTGGT750.002648729410.1332617
ACCGTAT856.598851E-410.0583588
AGGACCT20000.010.025375
Produced by FastQC (version 0.11.5)