FastQCFastQC Report
Thu 26 May 2016
SRR1512585_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512585_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108354
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT67790.3215304450770601No Hit
GTCCTAAAGTGTGTATTTCTCATTT62190.29496944061575997No Hit
CTGTAGGACGTGGAATATGGCAAGA57950.274858965809347No Hit
CTTTAGGACGTGAAATATGGCGAGG54370.2578788951001587No Hit
GTATCAACGCAGAGTACTTTTTTTT37960.1800456659555274No Hit
GTACATGGGAAGCAGTGGTATCAAC35130.16662287262954892No Hit
GTCCTACAGTGTGCATTTCTCATTT30750.14584837271160347No Hit
CTGAAGGACCTGGAATATGGCGAGA29380.1393504126916068No Hit
CCCATGTACTCTGCGTTGATACCAC26480.1255956068098621No Hit
GTCCTTCAGTGTGCATTTCTCATTT25290.11995139336183581No Hit
TATCAACGCAGAGTACTTTTTTTTT23570.11179337056300791No Hit
ATTTAGAAATGTCCACTGTAGGACG23370.11084476326081863No Hit
GGTATCAACGCAGAGTACTTTTTTT22540.10690804295673308No Hit
CTGTAGGACCTGGAATATGGCGAGA21790.10335076557352323No Hit
TTTCTAAATTTTCCACCTTTTTCAG21460.1017855635249109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG450.00803056813.3755441
TGGACCG500.001529610113.2653665
GCCTAAT852.1436335E-612.9821451
TCCAACG1750.012.97731418
TAGGACC10650.012.900594
GTGTAGG4000.012.037991
AAATGTC9100.011.4541587
GGCGAGG17500.011.14098419
GACGTGG18150.011.0158427
AGGACGT37450.010.9806765
GGACGTG36900.010.9389196
GTGTAGA3950.010.9205391
CTGTAGG33450.010.9163631
TGTAGGA36450.010.8925192
GACGTTG700.001523972610.8293867
AGGACCT22900.010.79931455
AATGTCC9900.010.6937328
TTAGGAC23550.010.6626913
AGGACCG803.863829E-410.6596685
CTGGGCG803.941977E-410.6365879
Produced by FastQC (version 0.11.5)