FastQCFastQC Report
Thu 26 May 2016
SRR1512585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512585_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108354
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT70380.33381490964041144No Hit
CTGTAGGACGTGGAATATGGCAAGA67350.31944350901224366No Hit
GTCCTAAAGTGTGTATTTCTCATTT66780.3167399782010042No Hit
CTTTAGGACGTGAAATATGGCGAGG64100.30402864035166766No Hit
GTATCAACGCAGAGTACTTTTTTTT48040.22785547398586764No Hit
GTACATGGGAAGCAGTGGTATCAAC41260.19569768644165067No Hit
CTGAAGGACCTGGAATATGGCGAGA34880.1654371135018123No Hit
GTCCTACAGTGTGCATTTCTCATTT34460.1634450381672148No Hit
TATCAACGCAGAGTACTTTTTTTTT32850.15580874938459102No Hit
GGTATCAACGCAGAGTACTTTTTTT30890.14651239782313596No Hit
CCCATGTACTCTGCGTTGATACCAC30280.14361914555145863No Hit
ATTTAGAAATGTCCACTGTAGGACG27450.13019635222548015No Hit
GTCCTTCAGTGTGCATTTCTCATTT26970.12791969470022585No Hit
TTTCTAAATTTTCCACCTTTTTCAG26960.1278722643351164No Hit
CTGTAGGACCTGGAATATGGCGAGA26400.12521616388898638No Hit
GAATATGGCAAGAAAACTGAAAATC24260.11506606575556097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2500.014.43417818
CGGTTTC2000.013.77369213
CCAACGT1450.013.752748519
CCAACGA1257.2759576E-1213.6741619
GCCGGTT1850.013.35103411
CGTGTAT655.4555006E-513.15353410
TAGGACC9700.013.0294824
CCGGTTT1900.012.99938312
TGCCGGT1950.012.17919810
CACCTTT8500.011.28448714
CTATACT2400.011.0864914
ACCGTGC600.005877872511.083078
ACCTTTT9000.011.07965315
CCTATAC1553.110472E-1011.03543
GGCGAGG16100.011.02945119
GTGTAGG2600.010.9758611
AGGACCT22200.010.744055
GGACCGT803.7693954E-410.6882616
AAATGTC8800.010.5797967
TGTAGGA35600.010.4903472
Produced by FastQC (version 0.11.5)