FastQCFastQC Report
Thu 26 May 2016
SRR1512584_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512584_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956039
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT64960.33209971784816156No Hit
GTCCTAAAGTGTGTATTTCTCATTT61590.31487102251028737No Hit
CTGTAGGACGTGGAATATGGCAAGA61040.3120592176331862No Hit
CTTTAGGACGTGAAATATGGCGAGG53030.2711091138775863No Hit
GTATCAACGCAGAGTACTTTTTTTT40350.20628423052914588No Hit
GTCCTACAGTGTGCATTTCTCATTT31630.16170434229583358No Hit
CTGAAGGACCTGGAATATGGCGAGA29960.1531666802144538No Hit
TATCAACGCAGAGTACTTTTTTTTT25330.12949639552176617No Hit
GTACATGGGAAGCAGTGGTATCAAC24910.1273491990701617No Hit
GGTATCAACGCAGAGTACTTTTTTT24270.12407728066771676No Hit
GTCCTTCAGTGTGCATTTCTCATTT23750.12141884696573024No Hit
ATTTAGAAATGTCCACTGTAGGACG23310.11916940306404933No Hit
TTTCTAAATTTTCCACCTTTTTCAG22980.11748232013778866No Hit
CTGTAGGACCTGGAATATGGCGAGA22250.1137502882099999No Hit
GAATATGGCAAGAAAACTGAAAATC20530.10495700750342912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9700.012.8963184
GGCGAGG15900.012.32415219
TCCAACG2750.011.35644618
GTCCTAA18950.011.1556541
CCTACAC2800.010.8312313
AGGACCT20700.010.8045145
GTATTGG1801.4915713E-1010.5755481
AAATGTC8000.010.5429857
GACAGTC1355.8960723E-810.5298237
CCCGTTT2250.010.52522412
ACCTTTT7850.010.50542515
CCACCTT8000.010.42345213
TGTAGGA35950.010.2813842
GTCCTAC34150.010.2683131
AGGACGT37400.010.2370155
GTAAGAC2500.010.2355143
CCAACGA2500.010.22368819
GGACCTG19750.010.2205926
AATGTCC8800.010.2054978
TTAGGAC23200.010.1717873
Produced by FastQC (version 0.11.5)