FastQCFastQC Report
Thu 26 May 2016
SRR1512584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512584_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956039
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA70690.3613936122950514No Hit
GTCCTACAGTGGACATTTCTAAATT69080.3531626925639008No Hit
GTCCTAAAGTGTGTATTTCTCATTT67710.3461587422336671No Hit
CTTTAGGACGTGAAATATGGCGAGG62240.3181940646377705No Hit
GTATCAACGCAGAGTACTTTTTTTT49790.2545450269652088No Hit
CTGAAGGACCTGGAATATGGCGAGA36060.18435215248775713No Hit
GTCCTACAGTGTGCATTTCTCATTT35950.18378979151233693No Hit
TATCAACGCAGAGTACTTTTTTTTT32560.16645884872438638No Hit
GGTATCAACGCAGAGTACTTTTTTT32120.16420940482270546No Hit
GTACATGGGAAGCAGTGGTATCAAC30350.1551605054909437No Hit
ATTTAGAAATGTCCACTGTAGGACG28210.14422002833276842No Hit
TTTCTAAATTTTCCACCTTTTTCAG27550.14084586248024705No Hit
CTGTAGGACCTGGAATATGGCGAGA25880.13230820039886731No Hit
GTCCTTCAGTGTGCATTTCTCATTT25630.13103010727291226No Hit
GAATATGGCAAGAAAACTGAAAATC23480.12003850638969878No Hit
CCCATGTACTCTGCGTTGATACCAC21320.1089957817814471No Hit
GGAATATGGCGAGAAAACTGAAAAT20040.10245194497655723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGC400.005280737314.2495988
TAGGACC10000.013.6838124
TTAGTCA1405.2750693E-1112.21768954
GGCGAGG14650.012.18548419
CCAACGT1052.7374517E-711.75646219
CACGTAT658.0528215E-411.68749318
GAAATGT9400.011.5224246
AAATGTC9000.011.5064087
TGTAGGA38650.011.3346252
AGGACCT23250.011.2405545
AATGTCC9650.011.2224828
TGTCCAC9750.011.20481210
GTCCTAG3000.011.0980631
TTTAGAA9950.011.0787472
TCCAACG2350.010.910398518
TAGAAAT9500.010.9030374
ATTTAGA9450.010.8715721
CTGTAGG38600.010.8434091
GACGTGG19500.010.8161867
GTAGGAC38750.010.6920113
Produced by FastQC (version 0.11.5)