FastQCFastQC Report
Thu 26 May 2016
SRR1512583_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512583_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818919
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT46220.25410697232806956No Hit
GTCCTAAAGTGTGTATTTCTCATTT41450.22788260499780363No Hit
CTGTAGGACGTGGAATATGGCAAGA39250.2157875089544944No Hit
GTATCAACGCAGAGTACTTTTTTTT34650.19049776268212054No Hit
CTTTAGGACGTGAAATATGGCGAGG34630.19038780726354498No Hit
GTCCTACAGTGTGCATTTCTCATTT26090.14343684353179004No Hit
GTACATGGGAAGCAGTGGTATCAAC20480.11259434862135147No Hit
GGTATCAACGCAGAGTACTTTTTTT20220.11116492817986948No Hit
TATCAACGCAGAGTACTTTTTTTTT19390.10660177830898462No Hit
CTGAAGGACCTGGAATATGGCGAGA19240.10577711266966808No Hit
CTGTAGGACCTGGAATATGGCGAGA19070.104842491611776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8650.013.8025774
AGGACCT17750.011.7443815
GGCGAGG13100.011.63558819
GGACCTG16950.011.5722666
TTTTACG752.1243472E-411.3706942
AATGTCC6450.011.137018
GTCCTAC22200.011.0095381
GTCTTAC1758.913048E-1110.8754641
CATTTCG700.001516963710.83522314
AGTACGT700.001523976710.8292335
GTCCTAA14100.010.7983321
CTTATAC1502.224624E-910.7392863
CACCTTT5950.010.67588114
GACCGTG803.860103E-410.6606167
TGTAGGA27150.010.6447572
TGGCGAG28250.010.62161718
GTAGGAC26250.010.5768433
GAAATGT7300.010.514296
GGGTATA1052.2903816E-510.4938691
GTCTAGG2200.010.4721871
Produced by FastQC (version 0.11.5)