FastQCFastQC Report
Thu 26 May 2016
SRR1512583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818919
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT49250.2707652182422637No Hit
CTGTAGGACGTGGAATATGGCAAGA45470.24998364413148688No Hit
GTCCTAAAGTGTGTATTTCTCATTT44280.24344129672624232No Hit
CTTTAGGACGTGAAATATGGCGAGG40950.22513371953341516No Hit
GTATCAACGCAGAGTACTTTTTTTT39310.216117375210221No Hit
GTCCTACAGTGTGCATTTCTCATTT28470.1565215383422791No Hit
GGTATCAACGCAGAGTACTTTTTTT25360.13942347075378286No Hit
TATCAACGCAGAGTACTTTTTTTTT24140.13271619022067505No Hit
CTGAAGGACCTGGAATATGGCGAGA23790.1307919703956031No Hit
GTACATGGGAAGCAGTGGTATCAAC22940.1261188651061427No Hit
CTGTAGGACCTGGAATATGGCGAGA22920.12600890968756717No Hit
ATTTAGAAATGTCCACTGTAGGACG18190.1000044531944523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1400.013.56553818
TAGGACC9150.013.0863624
CCAACGA1103.8380676E-812.08566119
GGCGAGG10950.011.53380419
CTACACT3450.011.0181934
CCGAGGG700.001492225410.8566099
TGTAGGA25700.010.649492
GTCCTAT2600.010.6119761
CCACCTT6200.010.57031513
CCGGTTT1002.403206E-510.44833812
AGGACCT17500.010.4266455
TGGCGAG24650.010.36263918
GTCCTAC25200.010.1560151
AGGACGT24250.010.0717015
GGACCTG16700.010.0700286
CTGTAGG25750.09.9760381
GAAATGT7050.09.9727866
GTAGGAC26700.09.9658943
ATTACAC2201.8189894E-129.9351553
AATGTCC5650.09.9198678
Produced by FastQC (version 0.11.5)