FastQCFastQC Report
Thu 26 May 2016
SRR1512582_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512582_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028301
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76320.376275513348364No Hit
CTGTAGGACGTGGAATATGGCAAGA69460.34245410321249165No Hit
GTCCTAAAGTGTGTATTTCTCATTT66740.3290438647912711No Hit
CTTTAGGACGTGAAATATGGCGAGG57440.2831926819540098No Hit
GTACATGGGAAGCAGTGGTATCAAC41360.20391450775797082No Hit
GTCCTACAGTGTGCATTTCTCATTT38840.19149031627948712No Hit
GTATCAACGCAGAGTACTTTTTTTT34950.17231170324325631No Hit
CTGAAGGACCTGGAATATGGCGAGA33300.16417681596567768No Hit
CCCATGTACTCTGCGTTGATACCAC31950.15752099910220427No Hit
CTGTAGGACCTGGAATATGGCGAGA27200.1341023842122052No Hit
GTCCTTCAGTGTGCATTTCTCATTT26180.1290735448042475No Hit
ATTTAGAAATGTCCACTGTAGGACG24530.12093865752666887No Hit
TTTCTAAATTTTCCACCTTTTTCAG23150.11413493362178494No Hit
GAATATGGCAAGAAAACTGAAAATC21840.10767632614685886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT1200.014.98859819
TCCAACG2350.014.50005118
GCACCGC400.005372294714.212756
TATGTCG1950.011.67071416
GGCGAGG16600.011.57648119
TAGGACC12300.011.4777644
TGTCGAG1803.6379788E-1211.04286218
GTCCTAC37200.010.9682281
CCGGTTT1900.010.96771112
AATGTCC8400.010.912348
AAATGTC8150.010.8121557
ACCGCCC700.001559047110.7998418
CACCTTT8700.010.79038114
GTCCTAT2700.010.7676081
GTGTAGG3450.010.7514651
GCCGGTT1951.8189894E-1210.68489811
ATGTCGA2050.010.63858917
TAGAAAT8950.010.5865164
AGGACCT24550.010.536285
GTCCTAA19300.010.4925021
Produced by FastQC (version 0.11.5)