FastQCFastQC Report
Thu 26 May 2016
SRR1512582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028301
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT84570.41694994973625704No Hit
CTGTAGGACGTGGAATATGGCAAGA82390.40620203806042593No Hit
GTCCTAAAGTGTGTATTTCTCATTT72610.3579843425605963No Hit
CTTTAGGACGTGAAATATGGCGAGG69350.34191177739398637No Hit
GTACATGGGAAGCAGTGGTATCAAC45990.22674149448232783No Hit
GTCCTACAGTGTGCATTTCTCATTT41620.2051963687835287No Hit
GTATCAACGCAGAGTACTTTTTTTT41070.20248473969100245No Hit
CTGAAGGACCTGGAATATGGCGAGA40670.20051264580552886No Hit
CCCATGTACTCTGCGTTGATACCAC35090.17300193610317205No Hit
CTGTAGGACCTGGAATATGGCGAGA31480.15520378878677277No Hit
ATTTAGAAATGTCCACTGTAGGACG28610.14105401515849966No Hit
GTCCTTCAGTGTGCATTTCTCATTT27850.1373070367760998No Hit
TTTCTAAATTTTCCACCTTTTTCAG27240.13429959360075255No Hit
GGTATCAACGCAGAGTACTTTTTTT26820.13222889502100527No Hit
TATCAACGCAGAGTACTTTTTTTTT25740.12690424153022653No Hit
GAATATGGCAAGAAAACTGAAAATC25050.12350237957778455No Hit
GGAATATGGCGAGAAAACTGAAAAT22980.11329679372045864No Hit
ATTCCAGGTCCTTCAGTGTGCATTT22010.10851446604818517No Hit
GAGTACATGGGAAGCAGTGGTATCA21460.10580283695565895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1058.913048E-1114.46879119
TAGGACC11000.013.8223644
ACGGCGA655.47398E-513.14874317
CGTGCGC550.003069417812.09068910
ACACCGT658.0181943E-411.6935376
TCCAACG2200.011.65456818
CACCTTT10350.011.65305714
CTAGGAC3600.011.6146243
CCACCTT10600.011.47035913
AATCCCG3400.011.450026519
TAGAAAT10350.011.3850854
ACTGTTC7350.011.1154458
GGACCTG24000.011.0053236
GCCGGTT1303.2694516E-810.96106911
GGCGAGG18200.010.955935519
ACCTTTT10900.010.89053715
CGGTTTC1408.36917E-910.85587413
AGGACCT25400.010.8502745
GAATACG803.775817E-410.68625113
TGTAGGA42950.010.6423352
Produced by FastQC (version 0.11.5)