FastQCFastQC Report
Thu 26 May 2016
SRR1512581_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512581_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772865
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31730.17897583854382595No Hit
GTCCTACAGTGGACATTTCTAAATT28080.15838769449450465No Hit
GTCCTAAAGTGTGTATTTCTCATTT26670.15043446624531479No Hit
CTGTAGGACGTGGAATATGGCAAGA26150.14750136079171286No Hit
GTACATGGGAAGCAGTGGTATCAAC23900.1348100391174737No Hit
CTTTAGGACGTGAAATATGGCGAGG22220.12533385226737512No Hit
TATCAACGCAGAGTACTTTTTTTTT19130.10790443716808668No Hit
GGTATCAACGCAGAGTACTTTTTTT19040.10739678430111711No Hit
CCCATGTACTCTGCGTTGATACCAC17790.10034605003765092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4100.014.5608724
GGACGGT400.00536834814.2141846
AACACCG400.00536834814.2141845
TAGACCG550.003127104412.060525
AAGTCGT1553.274181E-1011.004537
TTAGGCA1053.5725589E-610.8298544
TTAGGAC10050.010.5607553
ATCCTAC1801.5279511E-1010.5595371
GGACGTG14650.010.54346
GACGTGA8150.010.464437
AGTCGTC1451.5636033E-810.4289078
AAGGCGT1002.4664152E-510.4237366
AGGACGT15450.010.3654685
GTATAAG1956.002665E-1110.2602791
GTAGGAC14150.010.1795863
TTAATCC2151.8189894E-1210.1372484
GGCGAGG7500.010.09984519
TGTAGGA15300.010.0338272
GTCTTAG1707.1449904E-910.0037721
AGGACCT11100.09.9883465
Produced by FastQC (version 0.11.5)