##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512581_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1772865 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.85866718560071 31.0 25.0 33.0 2.0 34.0 2 27.42607023095385 31.0 26.0 34.0 16.0 34.0 3 28.498734534214393 31.0 28.0 34.0 16.0 34.0 4 32.25985170895697 35.0 33.0 37.0 19.0 37.0 5 32.541861337439684 35.0 33.0 37.0 25.0 37.0 6 32.74411418805154 37.0 35.0 37.0 25.0 37.0 7 32.704949333423585 37.0 35.0 37.0 25.0 37.0 8 32.71375203413683 37.0 35.0 37.0 25.0 37.0 9 34.121241042042115 39.0 35.0 39.0 25.0 39.0 10 34.02016679216974 39.0 35.0 39.0 23.0 39.0 11 34.06573766192011 39.0 35.0 39.0 23.0 39.0 12 33.97157595191963 39.0 35.0 39.0 23.0 39.0 13 33.93071440859851 39.0 35.0 39.0 23.0 39.0 14 34.96100549111184 40.0 36.0 41.0 22.0 41.0 15 34.86859461944367 40.0 35.0 41.0 19.0 41.0 16 34.71986304653767 40.0 35.0 41.0 18.0 41.0 17 34.64432768428504 40.0 35.0 41.0 17.0 41.0 18 34.58642141392605 40.0 35.0 41.0 17.0 41.0 19 34.579304684789875 40.0 35.0 41.0 18.0 41.0 20 34.36194464891574 39.0 34.0 41.0 13.0 41.0 21 34.3229681899073 39.0 34.0 41.0 11.0 41.0 22 34.218470667535314 39.0 34.0 41.0 10.0 41.0 23 34.04272688557786 39.0 34.0 41.0 2.0 41.0 24 33.89638635767529 39.0 34.0 41.0 2.0 41.0 25 33.74661917292067 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.7624872691189033 1101 2 -2.343298421630969 1101 3 -3.1917047466744854 1101 4 -3.8457681255401397 1101 5 -3.9268597957668803 1101 6 -3.878766601425511 1101 7 -3.8395679833882532 1101 8 -3.926619555845395 1101 9 -4.1140296419271785 1101 10 -4.798271454488653 1101 11 -4.659791708676941 1101 12 -4.793963325376975 1101 13 -4.788691834070814 1101 14 -5.693465238201107 1101 15 -5.617331786175441 1101 16 -6.057183209191582 1101 17 -5.783610071830527 1101 18 -6.215854048507726 1101 19 -5.98082470850844 1101 20 -5.844966182692787 1101 21 -6.2510860331528875 1101 22 -6.651188840092846 1101 23 -6.906911743532849 1101 24 -6.985202383948405 1101 25 -7.360557663807828 1102 1 0.0674330949311539 1102 2 -0.5061593566762035 1102 3 -1.053574295404104 1102 4 -1.8754768235813586 1102 5 -1.5519188068570955 1102 6 -1.5165952254803656 1102 7 -1.2892112081411824 1102 8 -1.395510069957755 1102 9 -1.7172448756884862 1102 10 -1.8721320567500328 1102 11 -1.9454266680076628 1102 12 -2.0189871232920424 1102 13 -2.143149202911413 1102 14 -2.353592315198 1102 15 -2.3245110173673496 1102 16 -2.480608096780294 1102 17 -2.372966405945519 1102 18 -2.436998592787326 1102 19 -2.3753631837392604 1102 20 -2.404594470868126 1102 21 -2.5058359422177645 1102 22 -2.4059039229405492 1102 23 -2.73725002218902 1102 24 -2.6625665036577217 1102 25 -2.8172725454373833 1103 1 2.1747137525389384 1103 2 0.6552209646185609 1103 3 0.04148805431350766 1103 4 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0.20961571977271376 2316 23 0.09715379009731606 2316 24 -0.13998285090728046 2316 25 0.11611316079095957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99638.0 3 7.0 4 924.0 5 3533.0 6 4235.0 7 3991.0 8 3594.0 9 3359.0 10 3405.0 11 3412.0 12 3355.0 13 3320.0 14 3585.0 15 3503.0 16 3629.0 17 3917.0 18 4446.0 19 5291.0 20 6723.0 21 8121.0 22 9642.0 23 11473.0 24 12953.0 25 15023.0 26 16600.0 27 18903.0 28 22160.0 29 26289.0 30 32246.0 31 40035.0 32 52151.0 33 68124.0 34 93474.0 35 133301.0 36 197177.0 37 264662.0 38 586664.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.325149196222156 19.802565159471197 11.728107433227597 25.144178211079048 2 15.049232960647545 20.064664187119167 40.517822218705135 24.368280633528155 3 18.80705609349047 24.526723713576363 29.38458399668592 27.281636196247245 4 12.550669518607785 16.645371293912874 37.85138071436039 32.95257847311895 5 13.51400519552132 37.59639906756325 35.55028118866477 13.339314548250664 6 32.35003665811405 36.45693251006614 18.049641360979827 13.143389470839983 7 28.375401906041347 31.27156076887309 22.284893543393956 18.068143781691603 8 25.697992158726386 36.36308604468282 20.40999790673071 17.52892388986009 9 26.974913369583902 14.943916418612647 19.860249079410462 38.22092113239299 10 15.374939936120297 28.413465615195456 34.40668192995845 21.804912518725796 11 34.53465438324655 22.093253973862026 24.450804608487182 18.92128703440424 12 23.81238283255203 24.374277773631924 31.322293304749564 20.49104608906648 13 29.316594660519428 21.64820855377618 25.45489395843829 23.5803028272661 14 21.39194408473244 21.69323508954473 27.282443352427915 29.63237747329492 15 23.471590944463216 30.51594328885478 22.91627403406721 23.096191732614795 16 22.654188930118554 27.631395463527618 27.381013116021634 22.33340249033219 17 22.35879952312898 28.239925306080103 27.410276309680448 21.990998861110466 18 22.29477633033871 26.957434588241146 29.177906868941317 21.56988221247883 19 23.984095944296243 26.610638875073473 27.20390423656011 22.201360944070174 20 24.157907556466803 26.83302712474943 27.548374080455908 21.460691238327858 21 23.33852594023679 26.32250384641037 27.011861979151945 23.327108234200892 22 23.803110326480763 27.529381685015665 26.700466611194486 21.967041377309084 23 22.667025347467316 27.40760248823502 27.415175475727725 22.51019668856993 24 23.559274465513575 26.627733542163657 27.82051078931525 21.992481203007518 25 23.24208047460544 26.81922650182279 27.378008740348253 22.560684283223512 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1380.0 1 1380.0 2 1286.0 3 1192.0 4 1192.0 5 1192.0 6 1840.5 7 2489.0 8 2489.0 9 2489.0 10 3186.0 11 3883.0 12 3883.0 13 3883.0 14 6022.0 15 8161.0 16 8161.0 17 8161.0 18 14054.5 19 19948.0 20 19948.0 21 19948.0 22 31412.0 23 42876.0 24 42876.0 25 42876.0 26 61236.0 27 79596.0 28 79596.0 29 79596.0 30 101889.5 31 124183.0 32 124183.0 33 124183.0 34 142101.0 35 160019.0 36 160019.0 37 160019.0 38 174854.5 39 189690.0 40 189690.0 41 189690.0 42 201931.5 43 214173.0 44 214173.0 45 214173.0 46 224167.0 47 234161.0 48 234161.0 49 234161.0 50 225804.0 51 217447.0 52 217447.0 53 217447.0 54 195523.5 55 173600.0 56 173600.0 57 173600.0 58 150576.5 59 127553.0 60 127553.0 61 127553.0 62 105578.0 63 83603.0 64 83603.0 65 83603.0 66 65581.5 67 47560.0 68 47560.0 69 47560.0 70 35587.5 71 23615.0 72 23615.0 73 23615.0 74 16949.0 75 10283.0 76 10283.0 77 10283.0 78 7213.0 79 4143.0 80 4143.0 81 4143.0 82 2926.0 83 1709.0 84 1709.0 85 1709.0 86 1241.0 87 773.0 88 773.0 89 773.0 90 567.5 91 362.0 92 362.0 93 362.0 94 313.0 95 264.0 96 264.0 97 264.0 98 233.0 99 202.0 100 202.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.5782589198839165 2 0.21011188105129266 3 0.19426183042702067 4 0.1867034432965849 5 0.16239251155615345 6 0.2152448155950961 7 0.4070247875613766 8 0.2986691034004281 9 0.25004723991956523 10 0.22082899713176132 11 0.21817792104869804 12 0.22725926678004246 13 0.2406274589435744 14 0.25241628663208987 15 0.33538932744456007 16 0.4942846748060343 17 0.5000944798391305 18 0.22094180887997678 19 0.19770258874759217 20 0.1903134192394796 21 0.20774283433876803 22 0.2827062410279406 23 0.1927952777002197 24 0.3136730659130842 25 0.35789527121354414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1772865.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.67469649272942 #Duplication Level Percentage of deduplicated Percentage of total 1 86.63127198193702 66.42426485994115 2 9.276882696227709 14.226043302638258 3 2.2710088266802795 5.2238673755406 4 0.7480777925600604 2.2943455078997452 5 0.3554497540706115 1.3627001005889725 6 0.19381840452455773 0.8916580404975308 7 0.1164693736130665 0.6251177710736127 8 0.08534832172998041 0.5235245331848057 9 0.05540339231079966 0.3823234461088365 >10 0.2394739567522178 3.0949330882335526 >50 0.012690944421969164 0.6835736163066729 >100 0.011151373746408852 1.7247912872313536 >500 0.0019195679264259963 1.0014056451837363 >1k 0.001033613498844765 1.5414514255711191 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3173 0.17897583854382595 No Hit GTCCTACAGTGGACATTTCTAAATT 2808 0.15838769449450465 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2667 0.15043446624531479 No Hit CTGTAGGACGTGGAATATGGCAAGA 2615 0.14750136079171286 No Hit GTACATGGGAAGCAGTGGTATCAAC 2390 0.1348100391174737 No Hit CTTTAGGACGTGAAATATGGCGAGG 2222 0.12533385226737512 No Hit TATCAACGCAGAGTACTTTTTTTTT 1913 0.10790443716808668 No Hit GGTATCAACGCAGAGTACTTTTTTT 1904 0.10739678430111711 No Hit CCCATGTACTCTGCGTTGATACCAC 1779 0.10034605003765092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 410 0.0 14.560872 4 GGACGGT 40 0.005368348 14.214184 6 AACACCG 40 0.005368348 14.214184 5 TAGACCG 55 0.0031271044 12.06052 5 AAGTCGT 155 3.274181E-10 11.00453 7 TTAGGCA 105 3.5725589E-6 10.829854 4 TTAGGAC 1005 0.0 10.560755 3 ATCCTAC 180 1.5279511E-10 10.559537 1 GGACGTG 1465 0.0 10.5434 6 GACGTGA 815 0.0 10.46443 7 AGTCGTC 145 1.5636033E-8 10.428907 8 AAGGCGT 100 2.4664152E-5 10.423736 6 AGGACGT 1545 0.0 10.365468 5 GTATAAG 195 6.002665E-11 10.260279 1 GTAGGAC 1415 0.0 10.179586 3 TTAATCC 215 1.8189894E-12 10.137248 4 GGCGAGG 750 0.0 10.099845 19 TGTAGGA 1530 0.0 10.033827 2 GTCTTAG 170 7.1449904E-9 10.003772 1 AGGACCT 1110 0.0 9.988346 5 >>END_MODULE