FastQCFastQC Report
Thu 26 May 2016
SRR1512581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512581_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772865
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35430.1998460119636859No Hit
CTGTAGGACGTGGAATATGGCAAGA31630.17841177980274867No Hit
GTCCTACAGTGGACATTTCTAAATT30450.17175588665803657No Hit
GTCCTAAAGTGTGTATTTCTCATTT30100.16978168106426605No Hit
GTACATGGGAAGCAGTGGTATCAAC27320.15410084806231722No Hit
CTTTAGGACGTGAAATATGGCGAGG26310.1484038547774365No Hit
GGTATCAACGCAGAGTACTTTTTTT23460.13232818065673357No Hit
TATCAACGCAGAGTACTTTTTTTTT22840.12883101646205436No Hit
CCCATGTACTCTGCGTTGATACCAC21220.11969326485660216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC551.9612118E-413.8175058
GTCGAGG751.4864883E-512.6603319
GGCGAGG7600.011.494246519
TAGGACC5500.011.4036254
GGACCGT1053.4661934E-610.8572246
TGGACCG803.7582137E-410.6915025
TAATACT1909.094947E-1210.5033394
TAGGACA5000.010.4533224
AGTAGGG1106.020682E-610.3672242
TGTCGAG1201.5318292E-610.28738818
TAATACC1303.8244616E-710.2340224
GTGTACG750.002618727510.1468671
GACCGAA750.002647101910.1336947
GTCCTAA8650.010.1175391
TTAGGAC10850.010.0723263
AGGACGT16350.010.0557525
TGGCGAG16550.010.04114118
ATAAGAC1803.9108272E-1010.0309663
GTCCTAC14650.09.9996851
TGTAGGA16800.09.9558272
Produced by FastQC (version 0.11.5)