FastQCFastQC Report
Thu 26 May 2016
SRR1512580_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512580_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994287
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT67550.33871754667206877No Hit
CTGTAGGACGTGGAATATGGCAAGA61630.3090327520562487No Hit
GTCCTAAAGTGTGTATTTCTCATTT61360.3076788847342434No Hit
CTTTAGGACGTGAAATATGGCGAGG52310.26229925782999136No Hit
GTATCAACGCAGAGTACTTTTTTTT34590.17344544691912447No Hit
GTCCTACAGTGTGCATTTCTCATTT32100.16095978161618663No Hit
CTGAAGGACCTGGAATATGGCGAGA27550.13814461007868978No Hit
ATTTAGAAATGTCCACTGTAGGACG24140.12104576723410423No Hit
GTACATGGGAAGCAGTGGTATCAAC23530.1179870299510552No Hit
GTCCTTCAGTGTGCATTTCTCATTT23510.1178867434827585No Hit
CTGTAGGACCTGGAATATGGCGAGA23160.11613173028756643No Hit
TTTCTAAATTTTCCACCTTTTTCAG21820.10941253691168823No Hit
GGTATCAACGCAGAGTACTTTTTTT20690.10374635145292527No Hit
TATCAACGCAGAGTACTTTTTTTTT20630.1034454920480352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8700.013.6141554
CGTGCGC707.5026164E-613.52919510
AAGTGCG655.586641E-513.1201877
GACCGTG854.059473E-612.2626587
CCAACGA1251.4952093E-912.11544119
CGGTTTC1501.4551915E-1112.00254113
TAGAAAT10550.011.8555414
CCGGTTT1701.8189894E-1211.70083812
ATAGGAC3250.011.6620933
TCCAACG2200.011.61490418
AATGTCC10550.011.376068
GCCGGTT1604.9112714E-1111.247560511
AAAGGCG1105.1392453E-711.198265
CAAGTCG1950.011.17645556
GTCCTAC31550.011.1619881
ATTTAGA10500.011.0841521
AGGACCT20000.010.9915255
TAGGACA9450.010.9293574
TAGGACT4000.010.896774
GTCCTAA18700.010.8915141
Produced by FastQC (version 0.11.5)