FastQCFastQC Report
Thu 26 May 2016
SRR1512580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512580_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994287
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72280.36243529642423583No Hit
CTGTAGGACGTGGAATATGGCAAGA72280.36243529642423583No Hit
GTCCTAAAGTGTGTATTTCTCATTT65830.3300929103985535No Hit
CTTTAGGACGTGAAATATGGCGAGG61220.30697687945616653No Hit
GTATCAACGCAGAGTACTTTTTTTT43290.2170700606281844No Hit
GTCCTACAGTGTGCATTTCTCATTT34210.17154000402148736No Hit
CTGAAGGACCTGGAATATGGCGAGA32540.1631660839187138No Hit
GGTATCAACGCAGAGTACTTTTTTT28250.14165463646907392No Hit
CTGTAGGACCTGGAATATGGCGAGA27880.13979933680558515No Hit
TATCAACGCAGAGTACTTTTTTTTT27280.13679074275668449No Hit
GTACATGGGAAGCAGTGGTATCAAC27140.13608873747860764No Hit
ATTTAGAAATGTCCACTGTAGGACG27070.13573773483956925No Hit
TTTCTAAATTTTCCACCTTTTTCAG26080.1307735546588831No Hit
GTCCTTCAGTGTGCATTTCTCATTT25060.12565894477575196No Hit
GAATATGGCAAGAAAACTGAAAATC23070.11568044118023134No Hit
GGAATATGGCGAGAAAACTGAAAAT20590.10324491911144183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1400.014.92179518
CCAACGA1300.014.6087519
TACGGCG400.005294843614.24388816
CGGCGAG655.4772798E-513.14787518
TAGGACC10000.013.0188854
GGCGAGG17400.011.89689519
TGGCGAA907.5012267E-611.60584118
ATACGGC855.344963E-511.17223715
AGGACCT20900.011.094225
TAGAAAT9000.010.9810544
TTAGGAC21950.010.952883
CACCTTT9800.010.75612814
TAGAACG803.7612964E-410.6906894
AAGTCGT2400.010.6885447
TAAAGGT2850.010.669854
AGGACGT39500.010.6094955
GACGTGA20300.010.5773887
AATCCCG2800.010.51308319
ACCTTTT10150.010.47878715
GGACGTG38900.010.4537256
Produced by FastQC (version 0.11.5)