FastQCFastQC Report
Thu 26 May 2016
SRR1512579_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512579_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188290
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72990.33354811291008046No Hit
CTGTAGGACGTGGAATATGGCAAGA62290.28465148586339106No Hit
GTCCTAAAGTGTGTATTTCTCATTT60160.2749178582363398No Hit
CTTTAGGACGTGAAATATGGCGAGG51730.23639462776871437No Hit
GTATCAACGCAGAGTACTTTTTTTT45360.20728514045213384No Hit
GTCCTACAGTGTGCATTTCTCATTT43300.19787139730108896No Hit
CTGTAGGACCTGGAATATGGCGAGA31530.1440851075497306No Hit
CTGAAGGACCTGGAATATGGCGAGA28010.1279994881848384No Hit
GGTATCAACGCAGAGTACTTTTTTT26300.1201851674138254No Hit
TATCAACGCAGAGTACTTTTTTTTT26230.11986528293781902No Hit
GTCCTTCAGTGTGCATTTCTCATTT22790.10414524583121981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCT250.006126859718.9491925
TAGGACC13450.012.8910854
TACACCG701.1189055E-412.1816225
AATGTCC7250.011.7362258
CCACCTT8300.011.53118113
GTGTAGG4550.011.4540211
GGCGAGG15900.011.25114219
AATGGCG855.436372E-511.15375616
GTCCTAC39200.011.0705171
TGAGCGA600.006006515611.05090210
GGACGTG38200.010.988066
GACGTGG20100.010.9834937
GGACCTG24600.010.977176
TAGGACT4500.010.9484214
AAATGTC7450.010.9376197
TGGCGAA1303.4526238E-810.92068718
TAGTACC1658.367351E-1110.9101394
AGGACGT38800.010.8664825
TGTAGGA44450.010.7854782
TCCTACA44150.010.7729262
Produced by FastQC (version 0.11.5)