FastQCFastQC Report
Thu 26 May 2016
SRR1512579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188290
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76320.34876547441152683No Hit
CTGTAGGACGTGGAATATGGCAAGA75010.34277906493197885No Hit
GTCCTAAAGTGTGTATTTCTCATTT64010.29251150441669066No Hit
CTTTAGGACGTGAAATATGGCGAGG61840.2825950856604929No Hit
GTATCAACGCAGAGTACTTTTTTTT53100.2426552239419821No Hit
GTCCTACAGTGTGCATTTCTCATTT48060.21962354166952278No Hit
CTGTAGGACCTGGAATATGGCGAGA38590.1763477418440883No Hit
CTGAAGGACCTGGAATATGGCGAGA34290.15669769546083928No Hit
GGTATCAACGCAGAGTACTTTTTTT34100.1558294375973934No Hit
TATCAACGCAGAGTACTTTTTTTTT33270.15203652166760345No Hit
TTTCTAAATTTTCCACCTTTTTCAG24930.11392457124055769No Hit
GAATATGGCAAGAAAACTGAAAATC24620.11250793998967229No Hit
GTCCTTCAGTGTGCATTTCTCATTT24090.11008595752848113No Hit
ATTTAGAAATGTCCACTGTAGGACG24070.10999456196390789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13600.014.3242134
CCAACGA1403.6379788E-1212.88735319
TCCAACG2150.012.80906818
CTAGGAC3600.011.8786163
AGGACCT25750.011.80968955
GGCGAGG15200.011.43261719
GGACCTG25050.011.26407056
TGTCGAG1701.2732926E-1111.1722118
CCGTTTC1553.1286618E-1011.03107613
GGTATCA12850.010.8091711
TGTAGGA41850.010.6950122
ATAGGAC4100.010.6617813
AATCCCG3250.010.51858419
CCACCTT8800.010.47046913
CACCTTT9100.010.43605714
CTGTAGG40500.010.4061811
TAGGACT4300.010.3868834
ATTGGAC1106.025768E-610.3667923
TTAGGAC22200.010.359013
GTAGGAC42050.010.3503563
Produced by FastQC (version 0.11.5)