FastQCFastQC Report
Thu 26 May 2016
SRR1512578_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512578_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988994
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43080.21659190525461616No Hit
GTCCTACAGTGGACATTTCTAAATT30720.15444993800886275No Hit
GTCCTAAAGTGTGTATTTCTCATTT28480.14318796336238318No Hit
GGTATCAACGCAGAGTACTTTTTTT26760.1345403756874078No Hit
TATCAACGCAGAGTACTTTTTTTTT26170.13157405200820113No Hit
CTGTAGGACGTGGAATATGGCAAGA25830.12986464514221763No Hit
CTTTAGGACGTGAAATATGGCGAGG24440.12287618766069681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1152.7284841E-1113.99566519
TCCAACG1700.013.36441518
TAGGACC6300.013.2363634
GGTATCA10750.011.82197951
GTGTAGG2850.011.2356991
GGCGAGG7700.011.18904819
GACCGAC600.005987012811.0556517
GCACGGT600.00598813911.0553716
GTCTTAA2200.010.9165041
GGACCTA803.860708E-410.6605386
TATACTG3200.010.6605385
GTATCAA23150.010.4174391
TAGGACT3650.010.3846944
TGTAGGA18900.010.3790382
AGGACCT11900.010.3519695
GTCCTAG2900.010.3518561
GCCGGTT1659.786163E-1010.33331111
ATAATAC2400.010.2657023
TGGCGAG17550.010.248589518
CGTGCGC750.002715543110.10266110
Produced by FastQC (version 0.11.5)