FastQCFastQC Report
Thu 26 May 2016
SRR1512578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988994
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48850.24560154530380687No Hit
TATCAACGCAGAGTACTTTTTTTTT32370.16274558897613567No Hit
CTGTAGGACGTGGAATATGGCAAGA31810.15993009531451577No Hit
GTCCTACAGTGGACATTTCTAAATT31480.1582709651210612No Hit
GGTATCAACGCAGAGTACTTTTTTT30820.15495270473415204No Hit
GTCCTAAAGTGTGTATTTCTCATTT29720.14942227075597012No Hit
CTTTAGGACGTGAAATATGGCGAGG29150.14655650042182128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.012.5355261
GAACCGT550.00306674412.0920776
ACAGCGC1354.7330104E-911.2589318
GGCGAGG8550.011.21738119
TAGACTG2850.011.0035545
TCCAACG1658.0035534E-1110.935518
GGACGTG18450.010.505086
CGGTTTC1451.4466423E-810.48139913
TGGCGAG18650.010.28588118
GACGTGG9300.010.2155287
GCCGGTT1409.694668E-810.17827511
CGAGTCA750.002648766910.133038510
TTAGGAC12100.010.1306073
AGGACGT18950.010.1299215
GTCCTAC17750.010.127171
TAGGACC5550.010.1023634
GTATCAA26000.010.0962561
TAGGACG20200.010.0205634
TCTATAC2201.8189894E-129.9342773
TGTAGGA18650.09.8844832
Produced by FastQC (version 0.11.5)