FastQCFastQC Report
Thu 26 May 2016
SRR1512577_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512577_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383954
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT83750.35130711414733673No Hit
CTGTAGGACGTGGAATATGGCAAGA73240.30722069301672766No Hit
GTCCTAAAGTGTGTATTTCTCATTT69820.2928747786240842No Hit
CTTTAGGACGTGAAATATGGCGAGG61270.2570099926424755No Hit
GTCCTACAGTGTGCATTTCTCATTT46670.1957672002060442No Hit
GTATCAACGCAGAGTACTTTTTTTT40610.17034724663311457No Hit
CTGTAGGACCTGGAATATGGCGAGA32780.13750265315522028No Hit
CTGAAGGACCTGGAATATGGCGAGA32760.13741875891900598No Hit
GTCCTTCAGTGTGCATTTCTCATTT26610.111621281283112No Hit
TATCAACGCAGAGTACTTTTTTTTT25450.10675541558268321No Hit
GGTATCAACGCAGAGTACTTTTTTT24940.10461611255921885No Hit
ATTTAGAAATGTCCACTGTAGGACG24370.10222512682711159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14450.013.9008274
GCGAGTA500.001556416413.2349299
TCCAACG1650.012.04290518
GCGTGCA951.1040102E-611.9412899
GGCGAGG17850.011.93101719
GGATCGT658.205106E-411.6616086
AATGTCC9150.011.5690878
GGCGTGC1501.9281288E-1011.3418378
AGGACCT27250.011.3350585
CTAATAC1701.2732926E-1111.1471253
GTCCTAC44150.011.1390491
GTCCTAT4150.011.1248061
GGACCTG26850.010.9748336
TGTAGGA45500.010.8494612
AGCGTGC700.001556925710.801758
GTAGGAC44800.010.7228883
GCGCCAC1159.10597E-710.71274113
GCGTGCG1159.397081E-710.6865879
ACCTTTT10300.010.67634215
CTGTAGG41200.010.6455211
Produced by FastQC (version 0.11.5)