##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512577_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2383954 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.7393473196211 31.0 25.0 33.0 2.0 34.0 2 27.393647276751146 31.0 26.0 34.0 16.0 34.0 3 28.51536145412202 31.0 28.0 34.0 16.0 34.0 4 32.27295577011972 35.0 33.0 37.0 20.0 37.0 5 32.57254628235276 35.0 33.0 37.0 25.0 37.0 6 32.7809097826552 37.0 35.0 37.0 25.0 37.0 7 32.75084796099254 37.0 35.0 37.0 25.0 37.0 8 32.74627782247476 37.0 35.0 37.0 25.0 37.0 9 34.1620194852753 39.0 35.0 39.0 25.0 39.0 10 34.06075620586639 39.0 35.0 39.0 25.0 39.0 11 34.10256531795496 39.0 35.0 39.0 25.0 39.0 12 34.01725284967747 39.0 35.0 39.0 23.0 39.0 13 33.986369703442264 39.0 35.0 39.0 23.0 39.0 14 35.001856998918605 40.0 36.0 41.0 22.0 41.0 15 34.93397481662817 40.0 36.0 41.0 22.0 41.0 16 34.79312981710218 40.0 35.0 41.0 19.0 41.0 17 34.728682264842355 40.0 35.0 41.0 18.0 41.0 18 34.681984216138396 40.0 35.0 41.0 18.0 41.0 19 34.690315752736836 40.0 35.0 41.0 18.0 41.0 20 34.48966674692549 40.0 34.0 41.0 17.0 41.0 21 34.44279923186437 39.0 34.0 41.0 16.0 41.0 22 34.345141726727945 39.0 34.0 41.0 11.0 41.0 23 34.174717297397514 39.0 34.0 41.0 10.0 41.0 24 34.05435339775851 39.0 34.0 41.0 2.0 41.0 25 33.91201969501089 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.16868055607358556 1101 2 -1.357597003738995 1101 3 -1.9487384752140642 1101 4 -2.1867973756078207 1101 5 -2.3487691958011823 1101 6 -2.6806096210591583 1101 7 -2.5888315200648755 1101 8 -2.772589624914705 1101 9 -2.955214151686185 1101 10 -3.4485487884444943 1101 11 -3.4756463212178446 1101 12 -3.1228932141551518 1101 13 -3.4451576804403956 1101 14 -4.465666069782348 1101 15 -4.17110790515245 1101 16 -4.290639820537031 1101 17 -4.682876893133546 1101 18 -4.541880381250984 1101 19 -4.475532244369099 1101 20 -4.235411342173144 1101 21 -4.337342709888233 1101 22 -4.58603032857922 1101 23 -5.088975420698183 1101 24 -5.41710236901039 1101 25 -5.70394662473397 1102 1 0.39658285624649636 1102 2 -0.29955084989746794 1102 3 -0.8016750251862881 1102 4 -1.512905693098272 1102 5 -1.3870462602106564 1102 6 -1.4326194188327044 1102 7 -1.4776215569899946 1102 8 -1.2922800638745677 1102 9 -1.6003546503031103 1102 10 -1.6374076708498606 1102 11 -1.7668407466889278 1102 12 -1.7501323840753642 1102 13 -1.81807791683093 1102 14 -2.1721052125132587 1102 15 -2.119958330000344 1102 16 -2.2383969587065096 1102 17 -2.127374172248281 1102 18 -2.1299344723237184 1102 19 -2.2775144167282235 1102 20 -2.286079089193521 1102 21 -2.61323112186151 1102 22 -2.507792207432228 1102 23 -2.6396360997029014 1102 24 -2.831628554805363 1102 25 -2.711153293812785 1103 1 2.350842564911048 1103 2 0.543832268907849 1103 3 -0.026797777190814998 1103 4 -0.27460440244760775 1103 5 -0.3874616820628418 1103 6 -0.5301550681754392 1103 7 -0.7557649660149153 1103 8 -0.79343162462051 1103 9 -0.9163426702334121 1103 10 -1.132287747551871 1103 11 -1.1878009646272645 1103 12 -1.1244100600015443 1103 13 -1.033316898112787 1103 14 -1.1602219798134072 1103 15 -1.2912297589626718 1103 16 -1.4466738037889826 1103 17 -1.3359154089971597 1103 18 -1.4346343184861468 1103 19 -1.2873745556688903 1103 20 -1.5041034224663221 1103 21 -1.4386128639822644 1103 22 -1.508806952598107 1103 23 -1.7669788888288664 1103 24 -1.7684517396801667 1103 25 -1.6287773437884994 1104 1 1.7110462566032112 1104 2 0.6727787144813426 1104 3 -0.46213024507656897 1104 4 -1.0313900388553776 1104 5 -1.0745200331538882 1104 6 -1.0827754219849623 1104 7 -1.2686436648703108 1104 8 -1.2441086692558265 1104 9 -1.4242640138176412 1104 10 -1.5046242061810986 1104 11 -1.3196164028426054 1104 12 -1.4096237678769015 1104 13 -1.3495972747521137 1104 14 -1.6629383008407927 1104 15 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1107 13 -0.6821630252322493 1107 14 -0.5846160034828927 1107 15 -0.761060590222705 1107 16 -0.9530249521998186 1107 17 -0.8829849138655135 1107 18 -0.7155934359647986 1107 19 -0.7761473153414897 1107 20 -0.5580265841912038 1107 21 -0.7812932792314555 1107 22 -0.8554811872361725 1107 23 -0.7775775384284529 1107 24 -0.8141933599118403 1107 25 -0.7326853786739917 1108 1 -3.1672224049640008 1108 2 -4.314635304763488 1108 3 -4.082478694927417 1108 4 -3.138390065938978 1108 5 -1.9832693512275483 1108 6 -1.3874635930295014 1108 7 -1.0785830362986637 1108 8 -1.0082982699275007 1108 9 -1.309943676742897 1108 10 -1.122667454820153 1108 11 -1.0871618936167664 1108 12 -1.1264099505983367 1108 13 -1.232330706360223 1108 14 -1.5786606397804306 1108 15 -1.4817823232956684 1108 16 -1.2936832671662586 1108 17 -1.302770144983782 1108 18 -1.396484639271975 1108 19 -1.302526430761688 1108 20 -1.3495517021009178 1108 21 -1.4372722058438399 1108 22 -1.48912734769997 1108 23 -1.3369147316633487 1108 24 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2315 16 0.6031060922181695 2315 17 0.5877638979089426 2315 18 0.5042841271631815 2315 19 0.49973574079534444 2315 20 0.625819879906615 2315 21 0.5588467725571533 2315 22 0.6390509950116652 2315 23 0.633985099735348 2315 24 0.7502564083434322 2315 25 0.8673697992843543 2316 1 0.2957148031906982 2316 2 0.10549859108072113 2316 3 -0.2223586051310562 2316 4 -0.08618300945113333 2316 5 -0.8277363493534615 2316 6 -0.6042396526579381 2316 7 -0.4285027957767724 2316 8 -0.3261513724374083 2316 9 -0.46394466370416865 2316 10 -0.364878133969313 2316 11 -0.46087289652345476 2316 12 -0.3412994879531084 2316 13 -0.40151868825262227 2316 14 -0.2878353290730402 2316 15 -0.3519206975479605 2316 16 -0.381791296638184 2316 17 -0.383010666875208 2316 18 -0.3399859556957523 2316 19 -0.24866652791746446 2316 20 -0.13257357213580434 2316 21 -0.2983681892537646 2316 22 -0.22555426264730016 2316 23 -0.3790196405120554 2316 24 -0.4475695263154904 2316 25 -0.32520065685468325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 132710.0 3 11.0 4 1124.0 5 4823.0 6 5645.0 7 5160.0 8 4737.0 9 4678.0 10 4401.0 11 4399.0 12 4425.0 13 4427.0 14 4465.0 15 4496.0 16 4941.0 17 5134.0 18 5637.0 19 6899.0 20 8746.0 21 10492.0 22 12961.0 23 14954.0 24 17025.0 25 19469.0 26 22059.0 27 25299.0 28 28932.0 29 33992.0 30 41944.0 31 52667.0 32 69064.0 33 91069.0 34 125865.0 35 181746.0 36 274904.0 37 360671.0 38 783983.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.96772351406329 20.75588193730243 12.269674181441443 25.00672036719284 2 13.649823191432478 20.145583316938815 43.668100976776955 22.53649251485175 3 18.61016882189494 24.761133011075326 29.79842833023642 26.830269836793313 4 11.61852128140202 17.062231421456865 39.53047479958393 31.788772497557183 5 12.390330191156666 38.56091529988635 36.65418059357517 12.39457391538182 6 31.164724478299615 38.13869410953606 18.389412971464125 12.307168440700195 7 27.6270857398415 31.394103604045586 22.909438368895962 18.06937228721695 8 23.706719006717115 39.475000683702426 20.795619255584796 16.022661053995662 9 26.22494019963733 15.086512873478918 20.985091121959094 37.70345580492466 10 14.486850049021175 29.372386027872277 35.814045785608464 20.326718137498087 11 33.64854013831375 21.891997561642107 26.346961511781892 18.11250078826225 12 23.642344595676946 24.479021483255742 33.11079409886325 18.76783982220406 13 29.41598945731584 22.584227518794084 25.836262090320933 22.163520933569146 14 19.640647045588832 23.558834846933305 28.146567222552815 28.65395088492505 15 21.76955267702282 33.8656612705752 23.05003537489178 21.314750677510197 16 20.4970981111587 28.43509616195271 30.457017984628926 20.61078774225966 17 20.631107866041972 29.897316606987935 29.194197996244753 20.277377530725342 18 20.402725979533294 27.587973876870205 32.5150658627056 19.494234280890904 19 23.334903759229757 26.973472690769974 27.963849664431788 21.727773885568485 20 23.27965082414881 28.109815906059392 29.176826167680726 19.433707102111075 21 21.60998694091721 27.35362962221969 27.657220722877184 23.379162713985913 22 22.442874598571155 29.94916811955692 27.7949227006303 19.813034581241627 23 20.69984495615777 29.402053605914613 29.021735978282937 20.87636545964468 24 22.80434434990448 27.224396360912635 29.993140943099284 19.978118346083605 25 21.72286599304374 28.41075282035047 29.312321978400398 20.55405920820539 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1749.0 1 1749.0 2 1665.5 3 1582.0 4 1582.0 5 1582.0 6 2469.0 7 3356.0 8 3356.0 9 3356.0 10 4573.0 11 5790.0 12 5790.0 13 5790.0 14 9449.0 15 13108.0 16 13108.0 17 13108.0 18 22702.5 19 32297.0 20 32297.0 21 32297.0 22 51866.5 23 71436.0 24 71436.0 25 71436.0 26 104507.0 27 137578.0 28 137578.0 29 137578.0 30 178696.0 31 219814.0 32 219814.0 33 219814.0 34 243507.5 35 267201.0 36 267201.0 37 267201.0 38 279826.5 39 292452.0 40 292452.0 41 292452.0 42 300529.0 43 308606.0 44 308606.0 45 308606.0 46 308551.0 47 308496.0 48 308496.0 49 308496.0 50 285086.0 51 261676.0 52 261676.0 53 261676.0 54 223561.0 55 185446.0 56 185446.0 57 185446.0 58 155737.0 59 126028.0 60 126028.0 61 126028.0 62 100966.5 63 75905.0 64 75905.0 65 75905.0 66 58166.0 67 40427.0 68 40427.0 69 40427.0 70 29429.5 71 18432.0 72 18432.0 73 18432.0 74 12968.5 75 7505.0 76 7505.0 77 7505.0 78 5194.5 79 2884.0 80 2884.0 81 2884.0 82 2011.5 83 1139.0 84 1139.0 85 1139.0 86 821.0 87 503.0 88 503.0 89 503.0 90 385.0 91 267.0 92 267.0 93 267.0 94 213.5 95 160.0 96 160.0 97 160.0 98 138.5 99 117.0 100 117.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.905021657297079 2 0.21434977352750934 3 0.1971934022216872 4 0.1899784979072583 5 0.16648811176725725 6 0.2178733314485095 7 0.3908212994042671 8 0.3015578320722631 9 0.2534444876033682 10 0.22609496659750983 11 0.22248751444029544 12 0.23058330823497436 13 0.24379665043872492 14 0.2545770597922611 15 0.33452826690447884 16 0.49103296456223566 17 0.4953115706091644 18 0.21250410033079498 19 0.19115301721425831 20 0.18490289661629378 21 0.1999619120167587 22 0.27303379175940473 23 0.19408931548175845 24 0.3160295878192281 25 0.3582283886350156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2383954.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.87005639186616 #Duplication Level Percentage of deduplicated Percentage of total 1 87.64157008083532 64.74087724142996 2 8.719369426190008 12.882006224283394 3 1.9665626172736084 4.358102743084121 4 0.6505357832692282 1.9222045998009882 5 0.3015465902431948 1.113763181301988 6 0.16527032497031569 0.732511693527556 7 0.10087443619080745 0.5216120202938856 8 0.06772636140275616 0.4002360108830004 9 0.05786098981926432 0.3846775122754424 >10 0.2835526525958382 3.8401585907963804 >50 0.022154693544750196 1.1358307952567652 >100 0.01868379401011329 2.9116187551170376 >500 0.001774129857313739 0.8757860916484093 >1k 0.0022891998158840665 2.9627324333228033 >5k 2.2891998158840665E-4 1.2178821069785437 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 8375 0.35130711414733673 No Hit CTGTAGGACGTGGAATATGGCAAGA 7324 0.30722069301672766 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6982 0.2928747786240842 No Hit CTTTAGGACGTGAAATATGGCGAGG 6127 0.2570099926424755 No Hit GTCCTACAGTGTGCATTTCTCATTT 4667 0.1957672002060442 No Hit GTATCAACGCAGAGTACTTTTTTTT 4061 0.17034724663311457 No Hit CTGTAGGACCTGGAATATGGCGAGA 3278 0.13750265315522028 No Hit CTGAAGGACCTGGAATATGGCGAGA 3276 0.13741875891900598 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2661 0.111621281283112 No Hit TATCAACGCAGAGTACTTTTTTTTT 2545 0.10675541558268321 No Hit GGTATCAACGCAGAGTACTTTTTTT 2494 0.10461611255921885 No Hit ATTTAGAAATGTCCACTGTAGGACG 2437 0.10222512682711159 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.194711810714469E-5 0.0 0.0 4.194711810714469E-5 0.0 9 4.194711810714469E-5 0.0 0.0 8.389423621428937E-5 0.0 10 4.194711810714469E-5 0.0 0.0 8.389423621428937E-5 0.0 11 4.194711810714469E-5 0.0 0.0 1.2584135432143405E-4 0.0 12 4.194711810714469E-5 0.0 0.0 1.2584135432143405E-4 0.0 13 4.194711810714469E-5 0.0 0.0 1.2584135432143405E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1445 0.0 13.900827 4 GCGAGTA 50 0.0015564164 13.234929 9 TCCAACG 165 0.0 12.042905 18 GCGTGCA 95 1.1040102E-6 11.941289 9 GGCGAGG 1785 0.0 11.931017 19 GGATCGT 65 8.205106E-4 11.661608 6 AATGTCC 915 0.0 11.569087 8 GGCGTGC 150 1.9281288E-10 11.341837 8 AGGACCT 2725 0.0 11.335058 5 CTAATAC 170 1.2732926E-11 11.147125 3 GTCCTAC 4415 0.0 11.139049 1 GTCCTAT 415 0.0 11.124806 1 GGACCTG 2685 0.0 10.974833 6 TGTAGGA 4550 0.0 10.849461 2 AGCGTGC 70 0.0015569257 10.80175 8 GTAGGAC 4480 0.0 10.722888 3 GCGCCAC 115 9.10597E-7 10.712741 13 GCGTGCG 115 9.397081E-7 10.686587 9 ACCTTTT 1030 0.0 10.676342 15 CTGTAGG 4120 0.0 10.645521 1 >>END_MODULE