FastQCFastQC Report
Thu 26 May 2016
SRR1512577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383954
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT90980.38163488053880235No Hit
CTGTAGGACGTGGAATATGGCAAGA87540.36720507190994456No Hit
GTCCTAAAGTGTGTATTTCTCATTT75370.3161554291735495No Hit
CTTTAGGACGTGAAATATGGCGAGG72640.304703865930299No Hit
GTCCTACAGTGTGCATTTCTCATTT52720.2211452066608668No Hit
GTATCAACGCAGAGTACTTTTTTTT51530.21615349960611654No Hit
CTGTAGGACCTGGAATATGGCGAGA41670.17479364115247192No Hit
CTGAAGGACCTGGAATATGGCGAGA38340.16082525082279273No Hit
TATCAACGCAGAGTACTTTTTTTTT33630.14106815819432758No Hit
GGTATCAACGCAGAGTACTTTTTTT32280.13540529724986303No Hit
ATTTAGAAATGTCCACTGTAGGACG29760.12483462348686258No Hit
TTTCTAAATTTTCCACCTTTTTCAG27980.11736803646379082No Hit
GTCCTTCAGTGTGCATTTCTCATTT27740.11636130562921936No Hit
GAATATGGCAAGAAAACTGAAAATC26910.11287969482632636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG456.74186E-414.7842275
TAAGACG759.636005E-713.9388284
TAGGACC15650.013.2384464
TCCAACG2250.011.8172118
ATAGGAC4350.011.5792793
AAATGTC8900.011.4217777
AATGTCC9750.011.3989938
ATACCGT600.0058737411.0842146
TACCGTA600.005875575411.0837497
TGTAGGA48100.010.9461032
ACCGTAT700.001493008610.8561838
CTGTAGG46700.010.7577831
AGGACGT45450.010.706545
AGGACCT29950.010.6941485
GGCGAGG19650.010.67972519
CACCTTT10000.010.63571214
GGACGTG45100.010.6172526
TTTAGAA11100.010.6167972
TCTATAC3050.010.5943413
CCAACGA1803.6379788E-1110.55085919
Produced by FastQC (version 0.11.5)