FastQCFastQC Report
Thu 26 May 2016
SRR1512576_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512576_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764848
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC51220.2902232940173885No Hit
CCCATGTACTCTGCGTTGATACCAC40990.2322579621587808No Hit
GTCCTACAGTGGACATTTCTAAATT37080.21010307969864828No Hit
GTCCTAAAGTGTGTATTTCTCATTT33460.18959139823939514No Hit
CTGTAGGACGTGGAATATGGCAAGA30860.17485925133495916No Hit
GTATCAACGCAGAGTACTTTTTTTT30500.17281941560972958No Hit
CTTTAGGACGTGAAATATGGCGAGG29310.1660766252957762No Hit
GAGTACATGGGAAGCAGTGGTATCA23010.13037950010425825No Hit
GGTATCAACGCAGAGTACTTTTTTT18500.10482489143540973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC456.903184E-414.7378233
TAGGACC5300.013.58542254
CCGTAGG500.001554071313.2372619
CGTGCGC655.611514E-513.11379710
TATGTCG1155.482434E-912.366961516
GTTCTAA1850.011.9259631
GATATAC2800.011.4613171
GGCGAGG9450.011.4182719
TAGACAG2300.011.1217035
ATAGGAC2150.011.0166443
AGACCCG700.001525996810.8274795
CCAACGA1053.6224756E-610.81730819
TAGGACA5550.010.754324
ACTGTTC4400.010.7408658
CGTTTCC803.8403337E-410.66650614
CCCGTTT803.8707917E-410.65738912
TGTAGGA20300.010.5480592
AGGACCT12000.010.5003995
GGACCTG11450.010.3431376
GTAGGAC20350.010.2425013
Produced by FastQC (version 0.11.5)