FastQCFastQC Report
Thu 26 May 2016
SRR1512576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764848
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC59400.33657289466288315No Hit
CCCATGTACTCTGCGTTGATACCAC47540.2693716399372637No Hit
GTCCTACAGTGGACATTTCTAAATT40360.228688249639629No Hit
GTCCTAAAGTGTGTATTTCTCATTT38590.21865905732391683No Hit
CTGTAGGACGTGGAATATGGCAAGA37920.21486269639085068No Hit
GTATCAACGCAGAGTACTTTTTTTT35910.20347361359165209No Hit
CTTTAGGACGTGAAATATGGCGAGG34770.19701413379509172No Hit
GAGTACATGGGAAGCAGTGGTATCA26180.1483413869069744No Hit
TATCAACGCAGAGTACTTTTTTTTT24300.13768891145299764No Hit
GGTATCAACGCAGAGTACTTTTTTT22900.12975621696599365No Hit
CTGAAGGACCTGGAATATGGCGAGA19430.11009446705891952No Hit
CATGTACTCTGCGTTGATACCACTG17750.10057523367451475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCCC550.003069550712.0904768
CGGTTTC802.8704233E-511.87356513
GGCGTGC1052.7217357E-711.7614848
TAGGACC5500.011.5762514
GGCGAGG9950.011.26177919
ACCGTCC600.0058777611.0829368
GGACCTG12350.010.6159076
GTATAAC1659.094947E-1010.37651
TTAGGAC12650.010.3667923
AGGACGT21200.010.3545675
GGACGTG20900.010.2732336
GACGTGA11100.010.1851867
TCCACGA750.0026505310.13210912
GTCCTAC18900.010.1157161
AGGACCT12500.010.1110775
TCCTACA19950.010.0985982
GGGCGTG951.6432919E-410.0004927
GATATAC3850.09.8823811
CTATAGT2700.09.8548514
TAGAAAT6300.09.8045714