FastQCFastQC Report
Thu 26 May 2016
SRR1512575_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512575_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767922
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT33810.19124146879783158No Hit
GTATCAACGCAGAGTACTTTTTTTT32950.18637700079528396No Hit
CTGTAGGACGTGGAATATGGCAAGA30500.17251892334616573No Hit
GTCCTAAAGTGTGTATTTCTCATTT29980.16957761711206717No Hit
CTTTAGGACGTGAAATATGGCGAGG23670.1338859972329096No Hit
GTACATGGGAAGCAGTGGTATCAAC21770.12313891676216483No Hit
GGTATCAACGCAGAGTACTTTTTTT21190.11985822903951644No Hit
TATCAACGCAGAGTACTTTTTTTTT20350.11510688819981876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC14050.012.0045221
GGCGAGG8300.011.97113919
GTATTAC1102.4291876E-711.8648581
TAGGACC5150.011.7747794
CTAACAC1657.2759576E-1211.485853
GACCGTC600.005989931511.0548157
CTATTAG1001.30208355E-511.0434461
GTATAGA2300.010.9124961
TCCTACA15850.010.8807592
TAGAACG700.001524693210.828594
AGGACCT12700.010.8179335
TCAATAC1159.111154E-710.71180253
GGTATAC750.00167276710.7087951
TGGCGAG18850.010.43974518
GGACCTG12550.010.2677476
TGTAGGA18950.010.2508942
TAGAAAT4450.010.2202434
TTAGGAC12450.010.1988813
AGGACGT20000.010.13826755
GTAGGAC17500.010.0714483
Produced by FastQC (version 0.11.5)