##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512575_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1767922 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.7290095377511 31.0 26.0 34.0 10.0 34.0 2 28.326834554918147 31.0 28.0 34.0 16.0 34.0 3 29.47832200741888 31.0 28.0 34.0 25.0 34.0 4 33.29850581643308 35.0 35.0 37.0 28.0 37.0 5 33.59229932089764 37.0 35.0 37.0 28.0 37.0 6 33.78578749514967 37.0 35.0 37.0 30.0 37.0 7 33.73746466190251 37.0 35.0 37.0 29.0 37.0 8 33.75998488621104 37.0 35.0 37.0 29.0 37.0 9 35.27356410520374 39.0 37.0 39.0 29.0 39.0 10 35.1964521059187 39.0 35.0 39.0 29.0 39.0 11 35.233647751427945 39.0 37.0 39.0 29.0 39.0 12 35.16581953276219 39.0 35.0 39.0 29.0 39.0 13 35.136718135754855 39.0 35.0 39.0 29.0 39.0 14 36.27304937661277 40.0 37.0 41.0 28.0 41.0 15 36.19906251520146 40.0 37.0 41.0 28.0 41.0 16 36.05287111082955 40.0 36.0 41.0 27.0 41.0 17 35.99439624598823 40.0 36.0 41.0 27.0 41.0 18 35.95046104975219 40.0 36.0 41.0 27.0 41.0 19 35.94718884656676 40.0 37.0 41.0 27.0 41.0 20 35.749916568717396 40.0 36.0 41.0 27.0 41.0 21 35.729901545430174 40.0 36.0 41.0 27.0 41.0 22 35.638274765515675 40.0 36.0 41.0 27.0 41.0 23 35.497587563252225 40.0 36.0 41.0 26.0 41.0 24 35.37821295283389 40.0 36.0 41.0 25.0 41.0 25 35.25056648426797 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0782644216705073 1101 2 -0.41957936880453417 1101 3 -1.087290744402793 1101 4 -1.3083085102550953 1101 5 -1.481819066373177 1101 6 -1.9661582069258472 1101 7 -1.7585760443833962 1101 8 -1.8117899649177502 1101 9 -2.0325335103132502 1101 10 -2.1232268707778985 1101 11 -2.206459412261239 1101 12 -2.0829952469470427 1101 13 -2.373605284665473 1101 14 -2.9543394993918994 1101 15 -2.7235653344853077 1101 16 -2.683953696490306 1101 17 -2.857622959146994 1101 18 -3.07651343931164 1101 19 -2.964232516731819 1101 20 -2.9177431024914284 1101 21 -3.228240398082157 1101 22 -2.9738731281466073 1101 23 -3.3036665461004944 1101 24 -3.4514021615568424 1101 25 -3.8522322744492463 1102 1 0.8257284374411604 1102 2 0.10806013509355239 1102 3 -0.5425345852301575 1102 4 -1.079432218735782 1102 5 -0.9087695948683319 1102 6 -1.1130323091775196 1102 7 -1.2091231136375598 1102 8 -1.168768131678526 1102 9 -1.4374625820043363 1102 10 -1.4748160710513147 1102 11 -1.553312216207864 1102 12 -1.5499048628841194 1102 13 -1.7291656282120869 1102 14 -1.9460938358258701 1102 15 -1.8100562653698375 1102 16 -1.9685510594629534 1102 17 -1.8553197703449484 1102 18 -2.057361062321732 1102 19 -1.9358042325827114 1102 20 -2.062888574868019 1102 21 -1.9952096988043166 1102 22 -2.288717799112561 1102 23 -2.337776029294645 1102 24 -2.65314540305981 1102 25 -2.599790399009656 1103 1 2.1583336239520214 1103 2 0.4101485526941282 1103 3 -0.24055622706167412 1103 4 -0.46634854485623833 1103 5 -0.6843472473803942 1103 6 -0.765275607515143 1103 7 -0.9183596520929598 1103 8 -0.7254391688211896 1103 9 -0.9790267657940177 1103 10 -1.2965197910601134 1103 11 -1.255989053004079 1103 12 -1.309169793482873 1103 13 -1.105182880426831 1103 14 -1.5224503582030309 1103 15 -1.565062374465846 1103 16 -1.7675370771298873 1103 17 -1.8248532459580105 1103 18 -1.6827801654490173 1103 19 -1.7388385232735928 1103 20 -1.823556364459911 1103 21 -1.8763494998040997 1103 22 -2.073537253791997 1103 23 -2.058985119884717 1103 24 -2.3411955008372445 1103 25 -2.150586017049193 1104 1 2.0139620485609413 1104 2 1.1440948636015165 1104 3 -0.013917899316115978 1104 4 -0.5314896071270141 1104 5 -0.5512782041674171 1104 6 -0.6321688823123495 1104 7 -0.8298348503750788 1104 8 -0.9099283842873263 1104 9 -0.9252909578379516 1104 10 -1.00130566186251 1104 11 -1.0783535720554198 1104 12 -1.1175682314869704 1104 13 -1.0770759874617468 1104 14 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-0.33248101358685744 2316 16 -0.28359489222637535 2316 17 -0.3041735454278509 2316 18 -0.15852045799335457 2316 19 -0.21000395538140992 2316 20 -0.19209798494131292 2316 21 -0.24293866683261456 2316 22 -0.17056997647215866 2316 23 -0.2666890358455518 2316 24 -0.25328868117444614 2316 25 -0.2888602729396865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66461.0 3 8.0 4 570.0 5 2628.0 6 3196.0 7 2910.0 8 2567.0 9 2544.0 10 2485.0 11 2461.0 12 2708.0 13 2617.0 14 2718.0 15 2817.0 16 2770.0 17 2952.0 18 3264.0 19 4135.0 20 5328.0 21 6059.0 22 7551.0 23 8850.0 24 10163.0 25 11506.0 26 13711.0 27 15324.0 28 17696.0 29 21446.0 30 26612.0 31 34123.0 32 44642.0 33 60480.0 34 84105.0 35 125595.0 36 208338.0 37 269193.0 38 687389.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.651086663716306 19.363563333397472 11.82582594139039 25.159524061495837 2 15.012821733688849 19.944427817380422 40.80774654912844 24.23500389980229 3 18.694420877659198 24.432739450345114 29.58318243978852 27.28965723220717 4 12.449875439475372 16.55725590557428 37.84577162921539 33.14709702573495 5 13.391046196729855 37.618551210723716 35.65742807609939 13.332974516447033 6 32.368945412699716 36.51955296178407 17.96843068951932 13.143070935996892 7 28.383325920081166 31.281197436207687 22.21129643294756 18.124180210763587 8 25.511148329892297 36.58384920493081 20.395608410763717 17.50939405441318 9 26.83256294264857 14.879853645618017 19.935567324546856 38.35201608718656 10 15.500678016834774 28.422851208185186 34.395295152746534 21.68117562223351 11 34.605662442765315 21.897167191323856 24.596189701979977 18.900980663930852 12 23.688760839592177 24.27002830085991 31.58578924930155 20.455421610246365 13 29.342844263247876 21.622093633139315 25.424121888085914 23.610940215526895 14 21.297860527997667 21.713823290398718 27.206346451897183 29.781969729706432 15 23.349459790182006 30.42162246836952 23.052622497882975 23.176295243565498 16 22.597400529991237 27.443619495627093 27.569664397631016 22.389315576750658 17 22.296287704599262 28.21798221973568 27.478900434613525 22.006829641051535 18 22.24632315840438 26.899309362665676 29.322606211994252 21.531761266935696 19 23.987904277147358 26.498248557468855 27.185958985637036 22.32788817974675 20 24.09583794732064 26.87358109264509 27.60061703697575 21.429963923058526 21 23.362420413938217 26.24735846831976 26.97825781349199 23.411963304250033 22 23.729052973144693 27.53043488126709 26.691249049796344 22.049263095791876 23 22.695825243488738 27.30972855525293 27.379717753681092 22.61472844757724 24 23.582177633520214 26.531311709830447 27.830764691195498 22.055745965453845 25 23.263238805444825 26.762988454328802 27.446543508299055 22.52722923192732 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1495.0 1 1495.0 2 1348.0 3 1201.0 4 1201.0 5 1201.0 6 1815.5 7 2430.0 8 2430.0 9 2430.0 10 3140.5 11 3851.0 12 3851.0 13 3851.0 14 6003.0 15 8155.0 16 8155.0 17 8155.0 18 14157.5 19 20160.0 20 20160.0 21 20160.0 22 31432.5 23 42705.0 24 42705.0 25 42705.0 26 61365.5 27 80026.0 28 80026.0 29 80026.0 30 102761.0 31 125496.0 32 125496.0 33 125496.0 34 142359.0 35 159222.0 36 159222.0 37 159222.0 38 173115.0 39 187008.0 40 187008.0 41 187008.0 42 199655.0 43 212302.0 44 212302.0 45 212302.0 46 221682.5 47 231063.0 48 231063.0 49 231063.0 50 223883.0 51 216703.0 52 216703.0 53 216703.0 54 195358.5 55 174014.0 56 174014.0 57 174014.0 58 151651.0 59 129288.0 60 129288.0 61 129288.0 62 107185.0 63 85082.0 64 85082.0 65 85082.0 66 66811.0 67 48540.0 68 48540.0 69 48540.0 70 36071.0 71 23602.0 72 23602.0 73 23602.0 74 16620.0 75 9638.0 76 9638.0 77 9638.0 78 6676.0 79 3714.0 80 3714.0 81 3714.0 82 2547.0 83 1380.0 84 1380.0 85 1380.0 86 928.5 87 477.0 88 477.0 89 477.0 90 341.5 91 206.0 92 206.0 93 206.0 94 155.0 95 104.0 96 104.0 97 104.0 98 82.0 99 60.0 100 60.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.930691512408353 2 0.21098215871514694 3 0.19418277503193013 4 0.18767796316805832 5 0.16188497003827093 6 0.21873136937036816 7 0.42767723915421607 8 0.3038029958335266 9 0.2562895874365498 10 0.22365240095434075 11 0.2213332941159169 12 0.22727247016553898 13 0.24531625264010518 14 0.25696835041364946 15 0.3401733786897838 16 0.5104863223603756 17 0.5157467354328981 18 0.22874312328258825 19 0.20713583517824882 20 0.19005363358790714 21 0.21460222792634517 22 0.29084993568720796 23 0.19027988791360703 24 0.3191317263996941 25 0.36178066679412324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1767922.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.87984774517581 #Duplication Level Percentage of deduplicated Percentage of total 1 84.08736592595494 61.2827442597648 2 10.73306327314751 15.644480343726574 3 2.7578488098634804 6.029748041011942 4 1.023273237393543 2.983039909718182 5 0.4633535353360693 1.6884567553740835 6 0.26499523486748533 1.158768742220366 7 0.16270132829337322 0.8300353623771233 8 0.1007985725212959 0.5876947694626485 9 0.07412384871096916 0.4861921327507691 >10 0.29928869623792126 3.6888212470948076 >50 0.016714944963866243 0.8697394179177013 >100 0.013116316880570932 1.9658791940159226 >500 0.002263534861406298 1.1256374873027422 >1k 0.0010927409675754534 1.658762337262378 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3381 0.19124146879783158 No Hit GTATCAACGCAGAGTACTTTTTTTT 3295 0.18637700079528396 No Hit CTGTAGGACGTGGAATATGGCAAGA 3050 0.17251892334616573 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2998 0.16957761711206717 No Hit CTTTAGGACGTGAAATATGGCGAGG 2367 0.1338859972329096 No Hit GTACATGGGAAGCAGTGGTATCAAC 2177 0.12313891676216483 No Hit GGTATCAACGCAGAGTACTTTTTTT 2119 0.11985822903951644 No Hit TATCAACGCAGAGTACTTTTTTTTT 2035 0.11510688819981876 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 5.656358142497237E-5 0.0 11 0.0 0.0 0.0 5.656358142497237E-5 0.0 12 0.0 0.0 0.0 5.656358142497237E-5 0.0 13 0.0 0.0 0.0 5.656358142497237E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAC 1405 0.0 12.004522 1 GGCGAGG 830 0.0 11.971139 19 GTATTAC 110 2.4291876E-7 11.864858 1 TAGGACC 515 0.0 11.774779 4 CTAACAC 165 7.2759576E-12 11.48585 3 GACCGTC 60 0.0059899315 11.054815 7 CTATTAG 100 1.30208355E-5 11.043446 1 GTATAGA 230 0.0 10.912496 1 TCCTACA 1585 0.0 10.880759 2 TAGAACG 70 0.0015246932 10.82859 4 AGGACCT 1270 0.0 10.817933 5 TCAATAC 115 9.111154E-7 10.7118025 3 GGTATAC 75 0.001672767 10.708795 1 TGGCGAG 1885 0.0 10.439745 18 GGACCTG 1255 0.0 10.267747 6 TGTAGGA 1895 0.0 10.250894 2 TAGAAAT 445 0.0 10.220243 4 TTAGGAC 1245 0.0 10.198881 3 AGGACGT 2000 0.0 10.1382675 5 GTAGGAC 1750 0.0 10.071448 3 >>END_MODULE