FastQCFastQC Report
Thu 26 May 2016
SRR1512575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767922
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40290.22789466956121368No Hit
CTGTAGGACGTGGAATATGGCAAGA35170.19893411587162785No Hit
GTCCTACAGTGGACATTTCTAAATT33590.1899970700064822No Hit
GTCCTAAAGTGTGTATTTCTCATTT31290.17698744627873855No Hit
CTTTAGGACGTGAAATATGGCGAGG28440.1608668255726214No Hit
TATCAACGCAGAGTACTTTTTTTTT26150.14791376542630275No Hit
GGTATCAACGCAGAGTACTTTTTTT25450.14395431472655468No Hit
GTACATGGGAAGCAGTGGTATCAAC24530.13875046523545723No Hit
CCCATGTACTCTGCGTTGATACCAC18840.10656578740464795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCC951.0360891E-612.0037723
TAGGACC3350.011.9141914
ACGAAAT1201.00444595E-811.87268412
GGCGAGG7050.011.85383119
ATTACTC1157.0440365E-811.5688523
TAGCACT1553.110472E-1011.0357274
CGAAATC1303.275636E-810.959413
CGAGACG909.551109E-510.55170518
TAGACTG1752.3464963E-1010.317825
ACCTTTT6050.010.04620615
TGGCGAG15400.09.98986218
CTAGCAC1054.090921E-59.9555093
TTAGGAC11200.09.757533
GTGTAGC3150.09.6650421
TAGAAAT5900.09.6640544
GTCTATA2201.6370905E-119.5140271
GTCTTAC1502.591405E-79.5140261
TTATACT2106.548362E-119.5029864
GACAACG800.0045148029.5002987
TCCAACG1304.2577067E-69.49653518
Produced by FastQC (version 0.11.5)