FastQCFastQC Report
Thu 26 May 2016
SRR1512574_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512574_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205214
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66160.3000162342520953No Hit
GTACATGGGAAGCAGTGGTATCAAC62380.28287504069899794No Hit
CTGTAGGACGTGGAATATGGCAAGA59730.27085806638267307No Hit
GTCCTAAAGTGTGTATTTCTCATTT56510.2562563089115161No Hit
CCCATGTACTCTGCGTTGATACCAC46670.21163478918599282No Hit
CTTTAGGACGTGAAATATGGCGAGG46660.21158944211310104No Hit
GTATCAACGCAGAGTACTTTTTTTT39110.17735240207979813No Hit
GTCCTACAGTGTGCATTTCTCATTT31290.14189099107841688No Hit
GAGTACATGGGAAGCAGTGGTATCA28070.12728923360725988No Hit
CTGAAGGACCTGGAATATGGCGAGA26350.11948953706987168No Hit
CTGTAGGACCTGGAATATGGCGAGA24440.11082824614753943No Hit
GGTATCAACGCAGAGTACTTTTTTT23660.10729117446197965No Hit
TATCAACGCAGAGTACTTTTTTTTT23570.10688305080595352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA450.007972049513.390391
CCAACGA2050.013.38819519
TCCAACG3350.013.27583118
TAGGACC10300.012.050554
GGCGAGG14400.011.76436419
CCAACGT1604.9112714E-1111.23858319
TGTAGGA35600.011.2050762
GACGTGG18350.011.0499527
AGGACCT20300.011.0151085
ACCGTGC951.4447831E-510.9407038
CTGTAGG33250.010.9036031
AGGACGT37050.010.8430245
GGACGTG36250.010.79480656
GGACCTG19750.010.7461696
GATATAC7200.010.7402091
GTCTTAT1705.9480953E-1010.6335451
GTCCTAA16650.010.6158051
GTAGGAC35850.010.5452413
GTCCTAC29400.010.45270251
TGTAGAA9250.010.3457582
Produced by FastQC (version 0.11.5)