FastQCFastQC Report
Thu 26 May 2016
SRR1512574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205214
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC74440.337563610606499No Hit
GTCCTACAGTGGACATTTCTAAATT71970.3263628836022264No Hit
CTGTAGGACGTGGAATATGGCAAGA68740.31171577905817754No Hit
GTCCTAAAGTGTGTATTTCTCATTT60230.27312542002726264No Hit
CTTTAGGACGTGAAATATGGCGAGG54950.24918216554039654No Hit
CCCATGTACTCTGCGTTGATACCAC54930.24909147139461296No Hit
GTATCAACGCAGAGTACTTTTTTTT50040.22691675275052672No Hit
GTCCTACAGTGTGCATTTCTCATTT33630.15250220613509619No Hit
GGTATCAACGCAGAGTACTTTTTTT33590.15232081784352902No Hit
TATCAACGCAGAGTACTTTTTTTTT33150.15032554663629016No Hit
CTGAAGGACCTGGAATATGGCGAGA32440.14710590446097296No Hit
GAGTACATGGGAAGCAGTGGTATCA32070.14542806276397663No Hit
CTGTAGGACCTGGAATATGGCGAGA28690.13010075212655098No Hit
GTCCTTCAGTGTGCATTTCTCATTT24210.1097852634710282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGA400.00527643514.2514646
TAGGACC9300.013.79330354
TCCAACG2600.012.41928118
CCAACGA1800.012.1346319
GTCGTAG657.94589E-411.7062551
GCACCGT658.0188486E-411.6935086
GGCGAGG12550.011.5776119
TGTAGGA34050.011.5531572
CTGTAGG33800.011.1715951
GTAGGAC33600.011.1422883
TCGTAGA600.005867534311.0857292
ACCGAGC600.00587844311.0829628
ATTAGAC1658.0035534E-1110.9417583
AGCCACG700.001491682610.8572737
CTGATCG700.001492247210.856789
ACTGTTC8700.010.7007918
ACCTTTT8800.010.68471915
AGGACGT31100.010.5716555
GACGTGG16450.010.5685157
AGGACCT21100.010.538065
Produced by FastQC (version 0.11.5)