FastQCFastQC Report
Thu 26 May 2016
SRR1512573_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512573_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026630
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55740.2750378707509511No Hit
GTCCTAAAGTGTGTATTTCTCATTT52300.2580638794451873No Hit
CTGTAGGACGTGGAATATGGCAAGA49930.24636958892348382No Hit
GTATCAACGCAGAGTACTTTTTTTT45360.2238198388457686No Hit
CTTTAGGACGTGAAATATGGCGAGG44970.22189546192447562No Hit
GTACATGGGAAGCAGTGGTATCAAC35060.17299655092444108No Hit
GTCCTACAGTGTGCATTTCTCATTT28690.141565061209989No Hit
GGTATCAACGCAGAGTACTTTTTTT28350.13988739927860538No Hit
TATCAACGCAGAGTACTTTTTTTTT27480.13559455845418256No Hit
CCCATGTACTCTGCGTTGATACCAC25470.12567661585982642No Hit
CTGAAGGACCTGGAATATGGCGAGA25180.12424566891835213No Hit
CTGTAGGACCTGGAATATGGCGAGA22380.11042962948342816No Hit
ATTTAGAAATGTCCACTGTAGGACG21570.10643284664689656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9500.013.6604684
GGTCGGT500.001533402613.26096511
TCCAACG1405.638867E-1112.16787218
CGTGCGC951.0788244E-611.9638910
ACCGTGC658.379092E-411.632318
GCGTGCG907.877232E-611.5549619
GTCCTAA14550.011.2490021
AGGACCT19050.011.1880955
AATGTCC7450.011.1638948
TCGTACA600.00600332311.0516272
CGAGCCT600.006004431311.05135254
ACCTTTT8850.011.03444815
GGACCTG18700.010.9424126
GGCGAGG13950.010.78868319
CGGTAGG750.001670477210.7107061
CACCTTT9050.010.6858314
CCACCTT9100.010.62157713
GTCTAAG1801.4370016E-1010.5991361
TATGTCG1701.4370016E-1010.59647316
GTCTTAG2850.010.5697761
Produced by FastQC (version 0.11.5)