FastQCFastQC Report
Thu 26 May 2016
SRR1512573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026630
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT60420.2981303938064669No Hit
CTGTAGGACGTGGAATATGGCAAGA58170.28702821926054584No Hit
GTCCTAAAGTGTGTATTTCTCATTT55590.274297725781223No Hit
GTATCAACGCAGAGTACTTTTTTTT55230.27252137785387565No Hit
CTTTAGGACGTGAAATATGGCGAGG54300.2679324790415616No Hit
GTACATGGGAAGCAGTGGTATCAAC41220.20339183768127386No Hit
TATCAACGCAGAGTACTTTTTTTTT36810.18163157557126855No Hit
GGTATCAACGCAGAGTACTTTTTTT35750.1764012177851902No Hit
GTCCTACAGTGTGCATTTCTCATTT30650.1512362888144358No Hit
CTGAAGGACCTGGAATATGGCGAGA30130.1486704529193785No Hit
CCCATGTACTCTGCGTTGATACCAC28210.13919659730685915No Hit
CTGTAGGACCTGGAATATGGCGAGA24590.12133443203742172No Hit
ATTTAGAAATGTCCACTGTAGGACG24150.11916334012621939No Hit
TTTCTAAATTTTCCACCTTTTTCAG22960.11329152336637668No Hit
GTCCTTCAGTGTGCATTTCTCATTT21140.10431109773367611No Hit
GAATATGGCAAGAAAACTGAAAATC20280.10006759990723517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACA604.0875105E-412.6686742
TAGGACC9750.012.4740794
ACCGTAT550.003069894912.0904378
GGCGAGG13750.011.53403219
TAGAAAT8900.010.5693524
GGACCTG18450.010.5556656
GTCGAGG1355.7192665E-810.55175919
CTACACT2900.010.4846794
CCACCTT7000.010.44856113
AGGACCT19200.010.4422755
AATGTCC8250.010.2480858
TATGTCG2053.6379788E-1210.19195716
CCTACAC2900.010.1567833
AACGTGG750.00264793710.1334387
ACTCCGG750.002649021810.1329388
TGTAGGA32250.010.1054782
TGTCCAC8850.010.09000210
ACCTTTT7250.010.08677315
ATTTAGA9150.010.0865791
TGTCGAG1901.0004442E-109.99689718
Produced by FastQC (version 0.11.5)