FastQCFastQC Report
Thu 26 May 2016
SRR1512572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118762
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT64280.30338471239336934No Hit
GTCCTAAAGTGTGTATTTCTCATTT59260.2796916312450384No Hit
CTGTAGGACGTGGAATATGGCAAGA57140.2696857882102851No Hit
CTTTAGGACGTGAAATATGGCGAGG50150.23669482461928237No Hit
GTATCAACGCAGAGTACTTTTTTTT48750.23008719242652076No Hit
GTCCTACAGTGTGCATTTCTCATTT38620.18227625377460988No Hit
GGTATCAACGCAGAGTACTTTTTTT30860.14565109247758834No Hit
GTACATGGGAAGCAGTGGTATCAAC29510.1392794471488539No Hit
TATCAACGCAGAGTACTTTTTTTTT28890.13635321003491663No Hit
CTGAAGGACCTGGAATATGGCGAGA26730.12615857750894155No Hit
CTGTAGGACCTGGAATATGGCGAGA25430.12002291904423433No Hit
GTCCTTCAGTGTGCATTTCTCATTT22670.10699644414993285No Hit
TTTCTAAATTTTCCACCTTTTTCAG22620.10676045728590564No Hit
ATTTAGAAATGTCCACTGTAGGACG22450.10595810194821316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11500.013.6765784
GTCTAAC602.5643833E-413.3872371
CCGGTTT1650.012.63028412
GTCCTAG3350.012.5879991
AGGACCT22550.011.5965735
GGACCTG22250.011.5825996
CGGTTTC1657.2759576E-1211.48643813
GCCGGTT1850.011.26431411
AATGTCC8300.011.1571178
GGCGAGG15650.011.00548419
AAATGTC8100.010.9956357
TCCAACG2500.010.97532718
TAGAAAT9250.010.9599654
TAAGACG803.86582E-410.6590784
GTCCTAC33350.010.6576061
TGGCGAG34550.010.59796218
GTGTAGG3150.010.5185431
ACTGTTC7300.010.4849458
GTTCTAG2500.010.4420451
TGTAGGA38900.010.4251412
Produced by FastQC (version 0.11.5)