FastQCFastQC Report
Thu 26 May 2016
SRR1512572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118762
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68510.32334920109007054No Hit
GTCCTAAAGTGTGTATTTCTCATTT66560.3141457133930097No Hit
CTGTAGGACGTGGAATATGGCAAGA65040.30697171272658275No Hit
GTATCAACGCAGAGTACTTTTTTTT62340.2942284220691139No Hit
CTTTAGGACGTGAAATATGGCGAGG58080.2741223412539964No Hit
GTCCTACAGTGTGCATTTCTCATTT41800.19728501832673986No Hit
TATCAACGCAGAGTACTTTTTTTTT40480.19105496511642178No Hit
GGTATCAACGCAGAGTACTTTTTTT39130.18468331978768732No Hit
GTACATGGGAAGCAGTGGTATCAAC34180.16132062024899446No Hit
CTGAAGGACCTGGAATATGGCGAGA32050.15126757984143568No Hit
CTGTAGGACCTGGAATATGGCGAGA28790.13588123630686222No Hit
TTTCTAAATTTTCCACCTTTTTCAG26660.12582819589930347No Hit
ATTTAGAAATGTCCACTGTAGGACG25580.12073087963631593No Hit
CCCATGTACTCTGCGTTGATACCAC24190.11417044481635975No Hit
GTCCTTCAGTGTGCATTTCTCATTT23420.11053624711034087No Hit
GAATATGGCAAGAAAACTGAAAATC22480.10609969406662947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC551.9602255E-413.8185618
TATTGCG802.8771152E-511.8711216
GGCGAGG16450.011.83420119
TAGGACC11850.011.62616354
CCAACGA1800.011.60649619
AATGTCC9100.011.5882288
ATTGCGA752.0812538E-411.39600717
AAATGTC9300.011.1347127
CACCTTT9550.011.03879914
TGGCGAG37050.011.02177818
ACCTTTT9300.010.72280915
AGGACCT24500.010.7035985
TCCAACG2400.010.68375618
TTAGGAC23200.010.6480993
GGACCTG24200.010.6392226
GTCCTAG3050.010.60522651
TAGAAAT10600.010.39773754
ATTTAGA10800.010.3943921
ATGTCCA10200.010.3385179
TTCCACC9650.010.3370711
Produced by FastQC (version 0.11.5)