FastQCFastQC Report
Thu 26 May 2016
SRR1512571_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512571_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912811
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT51460.2690281475796616No Hit
GTCCTAAAGTGTGTATTTCTCATTT47030.24586851497612675No Hit
CTGTAGGACGTGGAATATGGCAAGA45760.23922907176924432No Hit
CTTTAGGACGTGAAATATGGCGAGG40660.2125667407809763No Hit
GTATCAACGCAGAGTACTTTTTTTT34920.1825585486490824No Hit
GTCCTACAGTGTGCATTTCTCATTT26400.13801677217456404No Hit
GTACATGGGAAGCAGTGGTATCAAC23920.12505156024301406No Hit
CTGAAGGACCTGGAATATGGCGAGA21690.11339332532069296No Hit
GGTATCAACGCAGAGTACTTTTTTT21460.11219090647220244No Hit
TATCAACGCAGAGTACTTTTTTTTT20720.1083222545248851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8850.013.9178744
TGGCGAA1102.912202E-912.90398518
GCACCGT550.00313038112.0589146
GTCCTAT3450.011.9336971
TCCAACG1451.03682396E-1011.74707618
TGTCCAC8350.011.4551910
GTTCTAG1602.0736479E-1011.2969891
TATGTCG1105.1081224E-711.20345216
ATTTAGA7850.011.1290941
GGACCTG17500.011.0991226
GGACCGC600.005993509711.0540056
AGGACCT17700.011.0272395
GGCGAGG12950.010.9614919
AATGTCC7800.010.7830018
AAATGTC7950.010.726547
GTCTTAG1602.4774636E-910.6693791
TGGCGAG28700.010.61693818
GAAATGT8450.010.5400826
TAGAAAT8450.010.5400824
CTTAGAC1355.9069862E-810.5284533
Produced by FastQC (version 0.11.5)