FastQCFastQC Report
Thu 26 May 2016
SRR1512571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512571_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912811
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT53940.2819933595112115No Hit
CTGTAGGACGTGGAATATGGCAAGA51970.27169438067848833No Hit
GTCCTAAAGTGTGTATTTCTCATTT50010.2614476809261344No Hit
CTTTAGGACGTGAAATATGGCGAGG47920.2505213531289814No Hit
GTATCAACGCAGAGTACTTTTTTTT43400.22689120880212418No Hit
GGTATCAACGCAGAGTACTTTTTTT27950.14612002963178275No Hit
TATCAACGCAGAGTACTTTTTTTTT27930.14601547147104443No Hit
GTACATGGGAAGCAGTGGTATCAAC27920.14596319239067532No Hit
GTCCTACAGTGTGCATTTCTCATTT27740.14502216894403056No Hit
CTGAAGGACCTGGAATATGGCGAGA25130.1313773289676816No Hit
CTGTAGGACCTGGAATATGGCGAGA22600.11815072163428589No Hit
ATTTAGAAATGTCCACTGTAGGACG19840.10372169545239963No Hit
CCCATGTACTCTGCGTTGATACCAC19830.10366941637203048No Hit
GTCCTTCAGTGTGCATTTCTCATTT19560.10225788120206336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCA903.6476195E-813.72018312
TCCAACG1750.013.02493318
CCAACGA1251.03682396E-1012.91639119
TAGGACC9500.012.7027624
GGGTTAG1459.458745E-1111.8070951
CCGTCCA658.02787E-411.6917939
GCGCCAC1104.982794E-711.22501713
AGGACCT18950.011.1821425
GGCGAGG12700.010.99299119
GGACCTG18500.010.9901426
TGTAGGA28200.010.984372
ACACCGT700.001490595610.8580856
GGACCGC700.001490595610.8580856
GCGTGCG700.001492222410.8566669
CTGTAGG28000.010.7341471
GTAGGAC28550.010.6500233
TAGGACA7900.010.584584
AAATGTC6600.010.5076627
ACTGTTC5650.010.2561868
TGGCGAG29200.010.24542618
Produced by FastQC (version 0.11.5)