FastQCFastQC Report
Thu 26 May 2016
SRR1512570_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512570_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093529
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC56700.27083455734312734No Hit
GTCCTACAGTGGACATTTCTAAATT52220.24943528367650986No Hit
CTGTAGGACGTGGAATATGGCAAGA49700.23739819223903752No Hit
GTCCTAAAGTGTGTATTTCTCATTT47970.22913463343474105No Hit
CCCATGTACTCTGCGTTGATACCAC43140.20606354151291909No Hit
CTTTAGGACGTGAAATATGGCGAGG40640.19412198254717272No Hit
GTATCAACGCAGAGTACTTTTTTTT37310.1782158260047986No Hit
GTCCTACAGTGTGCATTTCTCATTT26850.12825234329211585No Hit
GAGTACATGGGAAGCAGTGGTATCA24490.1169795116284513No Hit
GGTATCAACGCAGAGTACTTTTTTT23190.11076990096626317No Hit
TATCAACGCAGAGTACTTTTTTTTT22940.10957574506968856No Hit
CTGAAGGACCTGGAATATGGCGAGA22900.1093846801262366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT655.593776E-513.1184686
CCAACGT1403.6379788E-1212.84707419
TAGGACC8000.012.6714684
TCCAACG2400.012.61865918
ACCGCTC751.5582487E-512.6026378
CCGGTTT1700.012.25810812
CGGTTTC1800.012.10740313
GACCGTG1201.0428266E-811.84306057
GATATAC4900.011.67719651
GCCGGTT1900.010.96646411
GTACCGT700.001525661110.8279426
GGCGAGG11700.010.67988919
GTAGGAC29050.010.4363193
TGTAGGA29900.010.2980662
AGGACCT18950.010.2489115
GTCCTAC25750.010.2137181
TGCCGGT2053.6379788E-1210.16330810
GTCCTAA14000.010.1099951
CCGCTCA750.002753674810.0857339
GTCTTAT2009.276846E-1110.0382931
Produced by FastQC (version 0.11.5)